Compound |
Cross-reactivity |
|---|---|
cAMP |
100% |
ADP |
<0.0001% |
ATP |
<0.0001% |
GTP |
<0.0001% |
cGMP |
<0.0001% |
GDP |
<0.0001% |
AMP |
<0.0001% |
GMP |
0.00817% |
Competitive ELISA Protocol:
- ELISA plate was coated with goat anti-mouse IgG antibody.
- THE™ cAMP Antibody(Mouse)(GenScript, A01509) at appropriate dulition and cAMP standards or testing compounds were added into appropriate reaction wells.
- After a period of incubation, cAMP-HRP conjugate (GenScript, M01059) was added and developed at room temperature.
- ELISA plate was washed with ELISA washing buffer, then TMB substrate was added and developed at room temperature.
- Stop the reaction with 1.0 N HCl and read the plate at 450 nm.
