Description
SUMO protease, also known as Ulp, is a protease
that specifically removes small ubiquitin-related modifier (SUMO) in any recombinant SUMO fusion protein. Different
from other tag removal proteases such as enterokinase (EK) and TEV which
recognize a specific amino acid sequence and cut at a specific cleavage site,
SUMO protease recognizes the SUMO tertiary structure and cleave it, more
specifically and leaving no residual amino acids.
GenScript’s SUMO Protease Animal-Free, His, is
the recombinant SUMO protease fragment from Saccharomyces
cerevisiae. This recombinant enzyme is expressed in E.coli and purified to obtain high yields of the active enzyme. It
is produced under an animal free process, and is suitable for drug and vaccine
development, manufacture and other applications. This product is designed with
a C-terminal 6x His tag which can be removed after the SUMO cleavage reaction
by purification using Ni2+ affinity chromatography resin (Cat. No. L00223) or Ni-charged magnetic beads (Cat. No. L00295).
|
| Source |
Recombinant SUMO Protease expressed in E.coli
|
| Species |
Saccharomyces
cerevisiae
|
| Tag |
His Tag
|
| Molecular Weight |
Predicted MW: 27 kDa
|
| Purity |
≥ 95% as analyzed by
SDS-PAGE
|
| Endotoxin Level |
< 0.2 EU/μg of
protein by gel clotting method
|
| Biological Activity |
10 U/μl
Unit definition: One
unit of SUMO Protease cleaves ≥ 85% of 2 μg control substrate
in 1 h at 30℃
|
| Storage Buffer |
25 mM Tris-HCl, 0.1% Igepal (NP-40), 250 mM NaCl, 0.5 mM
DTT, 50% (v/v) glycerol, pH 8.0 |
| Storage & Stability |
Upon receiving, the product remains stable for
up to 6 months at -20 °C. This product is stable for up to 1 week at 37 °C.
Avoid repeated freeze-thaw cycles by making single-use aliquots before the
solution is stored at -20 °C.
|
Lane 1: 2 μg of SUMO Protease Animal-Free, His, non-reducing (NR)
Lane 2: 2 μg of SUMO Protease Animal-Free, His, reducing (R)
> 95% as analyzed by SDS-PAGE
For
laboratory research or manufacture use only. Direct human use, including taking
orally and injection and clinical use are forbidden.
