| Product Description |
cGMP-peroxidase
conjugate is a product most suitable for Anti cGMP antibody in immunoassay
development for cGMP detection. HRP is conjugated to the C-8 position of cGMP. |
| Conjugation |
Horseradish peroxidase (HRP)
|
| Formulation |
Liquid, present in PBS, pH 7.4, containing 1% BSA and 0.01% |
| Storage |
The product
remains stable up to 1 year at -20 °C from date of receipt. It can be stored
for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid
repeated freezing and thawing cycles. |
| Note |
Small volumes
of the product will occasionally become entrapped in the seal of the product
vial during shipment and storage. Before use, briefly centrifuge the vial to
dislodge any liquid in the container's cap. |
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by the antibody affinity, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this product for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
|
| Application |
Recommended Usage |
| ELISA |
1:5,000 – 1:20,000 |
| Other |
user-optimized |
|
cGMP Competitive ELISA
1.ELISA plate was coated with goat anti mouse IgG antibody
2.Anti-cGMP monoclonal antibody (Cat. No. A01508) at appropriate dilution and cGMP standards or testing compounds were added into appropriate reaction wells.
3.After a period of incubation, HRP-cGMP conjugate (Cat.No.M01058) was added followed by proper period of incubation.
4.ELISA plate was washed with ELISA washing buffer, then TMB substrate was added and developed at room temperature.
5.Stop the reaction with 1.0 N HCl and read the plate at 450nm.
For research use only.
Not intended for human and animal therapeutic or diagnostic use.
