黄金城集团

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dsDNA_RAB11A_GFP

dsDNA_RAB11A_GFP
KIOK11
询价

联系黄金城集团
Description dsDNA_RAB11A_GFP HDR donor template is high purity GenWandTMclosed-end linear dsDNA designed for inserting GFP into targeted RAB11A locus. 

Advantages of GenWandTM dsDNA donor template:
✔ Covalently closed ends protection for better accuracy
✔ More suitable for scale up
✔ High purity and sequence verified
✔ Research to GMP grade
Learn More>>> //www.genscript.com/genwand-double-stranded-dna-synthesis-service.html

This dsDNA HDR donor template works in combination with SafeEdit Rab11a sgRNA and Cas9 protein.

[Reference] Roth. et al. Reprogramming human T cell function and specificity with non-viral genome targeting. Nature 2018, 559(7714): 405-409.
Sequence
GGTAGCTAGGAGTTCCAGGACTCAGTTTCCCCTTTGAGCCTCCTTTAGCGACTAAAGCTT
GAAGCCCCACGCATCTCGACTCTCGCGCACACCGCCCTTGTTGGGCTCAGGGGCGGGGCG
CCGCCCCCGGAAGTACTTCCCCTTAAAGGCTGGGGCCTGCCGGAAATGGCGCAGCGGCAG
GGAGGGGCTCTTCACCCAGTCCGGCAGTTGAAGCTCGGCGCTCGGGTTACCCCTGCAGCG
ACGCCCCCTGGTCCCACAGATACCACTGCTGCTCCCGCCCTTTCGCTCCTCGGCCGCGCA
ATGGGCGGATCGGGTGGGACTAGTGGCAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTG
CCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGCGCGGCGAG
GGCGAGGGCGATGCCACCAACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAG
CTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGC
CGCTACCCCGACCACATGAAGCGCCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTAC
GTCCAGGAGCGCACCATCAGCTTCAAGGACGACGGCACCTACAAGACCCGCGCCGAGGTG
AAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAG
GACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTTCAACAGCCACAACGTCTATATC
ACCGCCGACAAGCAGAAGAACGGCATCAAGGCCAACTTCAAGATCCGCCACAACGTGGAG
GACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCC
GTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGTGCTGAGCAAAGACCCCAAC
GAGAAGCGCGATCACATGGTCCTgCTGGAgTTCGTGACCGCCGCCGGGATCACTggaacc
ggtGCTggaagtggtACaCGCGACGACGAGTACGACTACCTCTTTAAAGGTGAGGCCATG
GGCTCTCGCACTCTACACAGTCCTCGTTCGGGGACCCGGGCCACTCCCGGTGGACCCTCG
TGCCGGCCACCCCTGCACTGATATAGGCCTCCCTCAGCCCTTCCTTTTTGTGCGGTTCCG
TCTCCTACCCAGCTCAGCCTCTTCTCCCCCGCTCAGACAGGGGTCCCCATCACATGCCGC
TCTCTGAGCGACCTCTCCATAGGCCTTCGCTGGCCTCAGAGCCCCTCCCTGCGTGTCCTT
CCCCTGGCGGACTGCCTTCTCCCACATCGT
Quantity 50μg
Storage Condition Stable for at least 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Quality Specifications
Item Specifications
Purity (Agarose gel electrophoresis) Single band
Sequence accuracy (Sanger sequencing) 100% sequence alignment
Optical density (Spectrophotometry) 260 nm/230 nm ≥ 2.0; 260 nm/280 nm = 1.8~2.0
Bioburden No colony formation
Endotoxin (Qualitative TAL assay) ≤ 10 EU/mg
Protein Residue (Micro BCA Protein Assay Kit ) ≤50μg/mg

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