黄金城集团

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ssDNA_TRAC_EGFP

ssDNA_TRAC_EGFP
KIOK5
询价

联系黄金城集团
Description ssDNA_TRAC_EGFP HDR donor template is high purity GenExactTM ssDNA designed for inserting EGFP into targeted TRAC locus. The resulting EGFP expression will be placed under the endogenous TCR promoter. 

This ssDNA HDR donor template works in combination with SafeEdit TRAC sgRNA and Cas9 protein.

Advantages of GenExactTM ssDNA donor template:
✔ Minimal cytotoxicity
✔ Precise knock-in, minimized off-target effect
✔ High purity and sequence verified
✔ Research to GMP grade
Learn More>>> //www.genscript.com/new-single-stranded-dna-synthesis-service.html
Sequence
GGTTTCCTTGAGTGGCAGGCCAGGCCTGGCCGTGAACGTTCACTGAAATCATGGCCTCTT
GGCCAAGATTGATAGCTTGTGCCTGTCCCTGAGTCCCAGTCCATCACGAGCAGCTGGTTT
CTAAGATGCTATTTCCCGTATAAAGCATGAGACCGTGACTTGCCAGCCCCACAGAGCCCC
GCCCTTGTCCATCACTGGCATCTGGACTCCAGCCTGGGTTGGGGCAAAGAGGGAAATGAG
ATCATGTCCTAACCCTGATCCTCTTGTCCCACAGATATCCAGAACCCTGACCCTGCCGTG
GGAAGCGGAGCTACTAACTTCAGCCTGCTGAAGCAGGCTGGAGACGTGGAGGAGAACCCT
GGACCTatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgag
ctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgcc
acctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctgg
cccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccac
atgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcacc
atcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgac
accctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctg
gggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcag
aagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcag
ctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgac
aaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcac
atggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtac
aagtaaTGATCATAATCAAGCCATATCACATCTGTAGAGGTTTACTTGCTTTAAAAAACC
TCCACACCTCCCCCTGAACCTGAAACATAAAATGAATGCAATTGTTGTTGTTAACTTGTT
TATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGC
ATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGT
CTGGATCTGCACCAGCTGAGAGACTCTAAATCCAGTGACAAGTCTGTCTGCCTATTCACC
GATTTTGATTCTCAAACAAATGTGTCACAAAGTAAGGATTCTGATGTGTATATCACAGAC
AAAACTGTGCTAGACATGAGGTCTATGGACTTCAAGAGCAACAGTGCTGTGGCCTGGAGC
AACAAATCTGACTTTGCATGTGCAAACGCCTTCAACAACAGCATTATTCCAGAAGACACC
TTCTTCCCCAGCCCAGGTAAGGGCAGCTTTGGTGCCTTCGCAGGCTGTTTCCTTGCTTCA
GGAATGGCCA
Quantity 50ug
Storage Condition Stable for at least 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Quality Specifications
Item Specifications
Purity (Agarose gel electrophoresis) Single band
Sequence accuracy (Sanger sequencing) 100% sequence alignment
Optical density (Spectrophotometry) 260 nm/230 nm ≥ 2.0; 260 nm/280 nm = 1.8~2.0
Bioburden No colony formation
Endotoxin (Qualitative TAL assay) ≤ 10 EU/mg
Protein Residue (Micro BCA Protein Assay Kit ) ≤50ug/mg

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