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Novel archaeal thermostable cellulases from an oil reservoir metagenome.

AMB Express. 2017-09; 
LewinAnna, ZhouJinglie, PhamVu Thuy Trang, HaugenTone, ZeinyMohamed El, AarstadOlav, LieblWolfgang, WentzelAlexander, LilesMa
Products/Services Used Details Operation
… Genes encoding four cellulase candidates were also synthesized as E. coli codon optimized variants (Genscript, Piscataway, NJ, USA), delivered cloned in vector pUC57 (//www.genscript.com/vector/SD1176-pUC57_plasmid_DNA.html) …

摘要

Microbial assemblages were sampled from an offshore deep sub-surface petroleum reservoir 2.5 km below the ocean floor off the coast of Norway, providing conditions of high temperature and pressure, to identify new thermostable enzymes. In this study, we used DNA sequences obtained directly from the sample metagenome and from a derived fosmid library to survey the functional diversity of this extreme habitat. The metagenomic fosmid library containing 11,520 clones was screened using function- and sequence-based methods to identify recombinant clones expressing carbohydrate-degrading enzymes. Open reading frames (ORFs) encoding carbohydrate-degrading enzymes were predicted by BLAST against the CAZy datab... More

关键词

CAZymes,Cellulase,Cellulose degradation,Enzyme discovery,Functional metagenomics,Oil reser