黄金城集团

至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

A Chaperone Lid Ensures Efficient and Privileged Client Transfer during Tail-Anchored Protein Targeting.

Cell Rep. 2019-01; 
ChioUn Seng,ChungSangYoon,WeissShimon,ShanSh
Products/Services Used Details Operation
Monoclonal Antibody Services … Key Resources Table. REAGENT or RESOURCE, SOURCE, IDENTIFIER. Antibodies. Rabbit polyclonal anti-Kar2p, Peter Walter Lab (UCSF), N/A. Mouse monoclonal anti-FLAG, GenScript, Cat#A00187; RRID: AB_1720813. Mouse monoclonal anti-PGK1, Abcam, Cat#ab197960 … Get A Quote

摘要

Molecular chaperones play key roles in maintaining cellular proteostasis. In addition to preventing client aggregation, chaperones often relay substrates within a network while preventing off-pathway chaperones from accessing the substrate. Here we show that a conserved lid motif lining the substrate-binding groove of the Get3 ATPase enables these important?functions during the targeted delivery of tail-anchored membrane proteins (TAs) to the endoplasmic reticulum. The lid prevents promiscuous TA handoff to off-pathway chaperones, and more importantly, it cooperates with the Get4/5 scaffolding complex to enable rapid and privileged TA transfer from the upstream co-chaperone Sgt2 to Get3. These findings pr... More

关键词

ATPase,chaperone,membrane protein,protein targeting,tail-anchored pro