黄金城集团

至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Carbon nanotubes as molecular transporters to study a new mechanism for molecular entry into the cell nucleus using actin polymerization force.

PLoS ONE. 2019; 
Sadr KarimiShaghayegh,PanteN
Products/Services Used Details Operation
Monoclonal Antibody Services 0221562 August 22, 2019 10 / 14 Carbon nanotubes and actin-based nuclear import To detect GST-VCA on the MWCNTs, a primary mouse monoclonal antibody against GST (GenScript, catalog number: A00865, dilution 1:500) and a secondary goat anti-mouse anti- body conjugated with 10 nm gold particles (Ted Pella Inc.... To detect GST-VCA, a mouse monoclonal antibody against GST (GenScript, catalog num- ber: A00865, dilution 1:500), and a goat anti-mouse Alexa Fluor 568 (Invitrogen, catalog num- ber: 1793903, dilution 1:600) were used as primary and secondary antibodies, respectively. Get A Quote

摘要

The transport of macromolecules into the cell nucleus occurs through nuclear pore complexes (NPCs) and is mediated by cellular receptors. Recently, a novel mechanism of nuclear entry, in which actin polymerization provides a propulsive force driving the transport through the NPC, has been proposed. This mechanism is used by the nucleocapsid from baculovirus, one of the largest viruses to replicate in the nucleus of their host cells, which crosses the NPC and enters the nucleus independently of cellular receptors. The baculovirus nucleocapsid contains a protein that hijacks the cellular actin polymerization machinery to assemble actin filaments that propel the nucleocapsid through the host cell cytopla... More

关键词