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A Quick HYL1-Dependent Reactivation of MicroRNA Production Is Required for a Proper Developmental Response after Extended Periods of Light Deprivation.

Dev. Cell. 2018; 
AchkarNatalia P,ChoSeok Keun,PoulsenChristian,ArceAgustin L,ReDelfina A,GiudicattiAxel J,KarayekovElizabeth,RyuMoon Young,ChoiSuk Won,HarholtJesper,CasalJorge J,YangSeong Wook,ManavellaPab
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Recombinant Antibody Services Developmental Cell 46, 236–247, July 16, 2018 247 STAR+METHODS KEY RESOURCES TABLE REAGENT or RESOURCE Antibodies Rabbit polyclonal anti-HYL1 Rabbit polyclonal anti-SE Rabbit polyclonal anti-ACTIN Rabbit polyclonal anti-H3 Goat polyclonal anti-Rabbit IgG, HRP conjugated Anti-Myc Anti-UTPase Mouse monoclonal anti-HIS-HRP Chemicals, Peptides, and Recombinant Proteins 1-aminocyclopropane-1-carboxylic acid (ACC) Jasmonic acid (JA) Abscicic acid (ABA) Indole-3-acetic acid (IAA) Gibberellin (GA3) 6-benzylaminopurine (BAP) Cycloheximide (CHX) 3-(3,4-diclorofenil)-1,1-dimetilurea (DCMU) Phos-tag Dexamethasone (Dex) SureBeads Protein-A Magnetic Beads Deposited Data SOURCE Agrisera Agrisera Agrisera Agrisera Agrisera Genscript Agrisera Sigma Sigma Sigma Sigma Sigma Sigma Sigma MP DuPont Wako Chemicals Sigma BioRad IDENTIFIER Cat#AS06136; RRID:AB_2233541 Cat#AS09532; RRID:AB_10507110 Cat#AS132640; RRID:AB_2722610 Cat# AS10710; RRID:AB_10750790 Cat#AS09602; RRID:AB_1966902 Cat#A00704; RRID:AB_914461 Cat#AS05086; RRID:AB_1031827 Cat#A7058; RRID:AB_258326 Cat#A3903 Cat#J2500 Cat#A1049 Cat#I2886 Cat#48870 Cat#B3408 Cat#100183 Cat# AAL-107 Cat#D1756 Cat#161-4013 Small RNA and mRNA sequencing data This paper European Nucleotide Archive (ENA): PRJEB20420 Experimental Models: Organisms/Strains Arabidopsis thaliana: Col-0 Arabidopsis thaliana: hyl1-2 Arabidopsis thaliana: cpl1-7 Arabidopsis thaliana: cpl2-2 Arabidopsis thaliana: cpl1-7/cpl2-2 Arabidopsis thaliana: HYL1S>D: Pro35S::eGFP-HYL1S>D in hyl1-2 Arabidopsis thaliana: HYL1S>A: Pro35S::eGFP-HYL1S>A in hyl1-2 Arabidopsis thaliana: HYL1S42D: Pro35S::eGFP-HYL1S42D in hyl1-2 Arabidopsis thaliana: HYL1S159D: Pro35S::eGFP-HYL1S159D in hyl1-2 Arabidopsis thaliana: HYL1S42A: Pro35S::eGFP-HYL1S42A in hyl1-2 Arabidopsis thaliana: HYL1S159A: Pro35S::eGFP-HYL1S159A in hyl1-2 Arabidopsis thaliana: HYL1WT: Pro35S::eGFP-HYL1WT in hyl1-2 N/A Arabidopsis Biological Resource Center N/A SALK_064863 Manavella et al. Get A Quote

摘要

Light is the most influential environmental stimulus for plant growth. In response to deficient light, plants reprogram their development to adjust their growth in search for a light source. A fine reprogramming of gene expression orchestrates this adaptive trait. Here we show that plants alter microRNA (miRNA) biogenesis in response to light transition. When plants suffer an unusual extended period of light deprivation, the miRNA biogenesis factor HYPONASTIC LEAVES 1 (HYL1) is degraded but an inactive pool of phosphorylated protein remains stable inside the nucleus. Degradation of HYL1 leads to the release of gene silencing, triggering a proper response to dark and shade. Upon light restoration, a qui... More

关键词

Arabidopsis,HYL1,gene silencing,light response,microRNAs,phosphorylation,s