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The InR/Akt/TORC1 Growth-Promoting Signaling Negatively Regulates JAK/STAT Activity and Migratory Cell Fate during Morphogenesis.

Dev Cell. 2018; 
Kang D, Wang D, Xu J, Quan C, Guo X, Wang H, Luo J, Yang Z, Chen S, Chen J.
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Monoclonal Antibody Services 8 Developmental Cell 44, 1–8, February 26, 2018 STAR+METHODS KEY RESOURCES TABLE REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-beta-galactosidase Developmental Studies Hybridoma Bank Cat# 40-1a; RRID: AB_528100 Mouse monoclonal anti-Singed (fascin) Developmental Studies Hybridoma Bank Cat# sn 7C; RRID: AB_528239 Mouse monoclonal anti- Fasciclin III Developmental Studies Hybridoma Bank Cat# 7G10; RRID: AB_528238 Mouse monoclonal anti-alpha-tubulin Developmental Studies Hybridoma Bank Cat# 12G10; RRID: AB_1157911 Mouse monoclonal anti-HA-probe Santa Cruz Biotechnology Cat# sc-7392; RRID: AB_627809 Mouse monoclonal anti-c-Myc Santa Cruz Biotechnology Cat# sc-40; RRID: AB_627268 Rabbit polyclonal anti-GFP (FL) Santa Cruz Biotechnology Cat# sc-8334; RRID: AB_641123 Protein A/G PLUS-Agarose antibody Santa Cruz Biotechnology Cat# sc-2003; RRID: AB_10201400 Mouse monoclonal anti-FLAG M2 Sigma-Aldrich Cat# F1804; RRID: AB_262044 Rabbit polyclonal anti-phospho-Drosophila Akt (Ser505) Cell Signaling Technology Cat# 4054S; RRID: AB_331414 Mouse monoclonal anti-beta-Actin GenScript Cat# A00702; RRID: AB_914102 ChromoTek GFP-Trap agarose beads ChromoTek Cat# gta-100; RRID: N/A Mouse monoclonal anti-Multi Ubiquitin MBL International Cat# D058-3; RRID: AB_592937 Cy3-AffiniPure Goat Anti-Mouse IgG (H+L) Jackson ImmunoResearch Labs Cat# 115-165-166; RRID: AB_2338692 Alexa Fluor 647-AffiniPure Goat Anti-Rat IgG (H+L) Jackson ImmunoResearch Labs Cat# 112-605-167; RRID: AB_2338404 Alexa Fluor 647-AffiniPure Goat Anti-Rabbit IgG (H+L) Jackson ImmunoResearch Labs Cat# 111-605-144; RRID: AB_2338078 Peroxidase-AffiniPure Goat Anti-Mouse IgG Jackson ImmunoResearch Labs Cat# 115-035-003; RRID: AB_10015289 Peroxidase-AffiniPure Goat Anti-Rabbit IgG (H+L) Jackson ImmunoResearch Labs Cat# 111-035-003; RRID: AB_2313567 Neutravidin HRP Thermo Fisher Cat# A2664; RRID: N/A Rabbit polyclonal anti-Slbo Gift from Pernille Rorth (Montell et al.... Primary antibodies used for western blot were mouse anti-GFP (1:5000 Santa Cruz), mouse anti-beta-actin (1:10000 Genscript), mouse anti-alpha-tubulin (1:10000 Santa Cruz), mouse anti-HA (1:5000, Santa Cruz) and mouse anti-Myc (1:5000, Santa Cruz), rabbit anti-p-dAkt (1:5000, CST), rabbit anti-t-dAkt (1:5000, from Tien Hsu) and mouse anti-Flag (1:50000, Sigma), and mouse anti- Multi-Ubiquitin (MBL International). Get A Quote

摘要

Cell growth and cell differentiation are two distinct yet coupled developmental processes, but how they are coordinated is not well understood. During Drosophila oogenesis, we found that the growth-promoting InR/Akt/TOR pathway was involved in suppressing the fate determination of the migratory border cells. The InR/Akt/TOR pathway signals through TOR and Raptor, components of TORC1, to downregulate the JAK/STAT pathway, which is necessary and sufficient for border cell fate determination. TORC1 promotes the protein stability of SOCS36E, the conserved negative regulator of JAK/STAT signaling, through physical interaction, suggesting that TORC1 acts as a key regulator coordinating both cell growth and cell dif... More

关键词

SOCS36E; STAT; TORC1; cell growth; cell migration; fate determination