黄金城集团

至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

The AMSH3 ESCRT-III-Associated Deubiquitinase Is Essential for Plant Immunity.

Cell Rep. 2018; 
Schultz-Larsen T, Lenk A, Kalinowska K, Vestergaard LK, Pedersen C, Isono E, Thordal-Christensen H.
Products/Services Used Details Operation
Monoclonal Antibody Services (2007) N/A Anti-HA Rat monoclonal Chromotek Cat# 7C9 Anti-GST Mouse monoclonal Genscript Cat# A00865-40 Anti-Gal4-BD (GBD) Mouse monoclonal Clontech Cat# 630403 Anti-Rat AP-conjugated Sigma-Aldrich Cat# A3687 Anti-Rabbit HRP-conjugated Santa Cruz Biotechnology Cat# sc-2004 Anti-Mouse HRP-conjugated Sigma-Aldrich Cat# A4416 Anti-Goat AP-conjugated Santa Cruz Biotechnology Cat# sc-2771 Bacterial and Virus Strains Pst DC3000 AvrRpm1 Grant et al. Get A Quote

摘要

Plant "nucleotide-binding leucine-rich repeat" receptor proteins (NLRs) detect alterations in host targets of pathogen effectors and trigger immune responses. The Arabidopsis thaliana mutant pen1 syp122 displays autoimmunity, and a mutant screen identified the deubiquitinase "associated molecule with the SH3 domain of STAM3" (AMSH3) to be required for this phenotype. AMSH3 has previously been implicated in ESCRT-mediated vacuolar targeting. Pathology experiments show that AMSH3 activity is required for immunity mediated by the CC-NLRs, RPS2 and RPM1. Co-expressing the autoactive RPM1D505V and the catalytically inactive ESCRT-III protein SKD1E232Q in Nicotiana benthamiana supports the requirement of ESCRT-asso... More

关键词

AMSH3 deubiquitinase; ESCRT; autoimmunity; nucleotide-binding leucine-rich repeat receptors; plant immunity