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Molecular analysis of PRC2 recruitment to DNA in chromatin and its inhibition by RNA.

Nat Struct Mol Biol. 2017; 
Wang X,, Paucek RD,, Gooding AR,, Brown ZZ, Ge EJ, Muir TW, Cech TR,.
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Catalog Antibody Then, the concentrated eluate was incubated with equilibrated Anti-FLAG G1 affinity resin (GenScript L00432-25) in the cold room with slow agitation for 2–3 h. Get A Quote

摘要

Many studies have revealed pathways of epigenetic gene silencing by Polycomb repressive complex 2 (PRC2) in vivo, but understanding the underlying molecular mechanisms requires biochemistry. Here we analyze interactions of reconstituted human PRC2 with nucleosome complexes. Histone modifications, the H3K27M cancer mutation, and inclusion of JARID2 or EZH1 in the PRC2 complex have unexpectedly minor effects on PRC2-nucleosome binding. Instead, protein-free linker DNA dominates the PRC2-nucleosome interaction. Specificity for CG-rich sequences is consistent with PRC2 occupying CG-rich DNA in vivo. PRC2 preferentially binds methylated DNA regulated by its AEBP2 subunit, suggesting how DNA and histone methylation c... More

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