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Glycoproteomic measurement of site-specific polysialylation

Anal Biochem. 2020; 
Pelingon R,  Pegg CL,  Zacchi LF,  Phung TK,  Howard CB,  Xu P,  Hardy MP,  Owczarek CM,  Schulz BL.
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Proteins, Expression, Isolation and Analysis Expression of secreted polysialylated rHuNCAM was verified by SDS-PAGE and Coomassie Blue staining, and Western blot analysis using antiHis (A01620, Genscript) and anti-PSA-NCAM (MAB5324, Millipore) antibodies. Get A Quote

摘要

Polysialylation is the enzymatic addition of a highly negatively charged sialic acid polymer to the non-reducing termini of glycans. Polysialylation plays an important role in development, and is involved in neurological diseases, neural tissue regeneration, and cancer. Polysialic acid (PSA) is also a biodegradable and non-immunogenic conjugate to therapeutic drugs to improve their pharmacokinetics. PSA chains vary in length, composition, and linkages, while the specific sites of polysialylation are important determinants of protein function. However, PSA is difficult to analyse by mass spectrometry (MS) due to its high negative charge and size. Most analytical approaches for analysis of PSA measure its de... More

关键词

Glycoproteomics; Mass spectrometry; N-linked glycosylation; Polysialylation; Sialic acid