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Molecular characterization of differences between the tomato immune receptors Fls3 and Fls2

biorxiv. 2020; 
Robyn Roberts,  Alexander E. Liu,  Lingwei Wan,  Annie M. Geiger,  Sarah R. Hind, Hernan G. Rosli,   Gregory B. Martin
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Molecular Biology Tools For the transiently-expressed proteins in N. benthamiana, total protein was extracted from Agroinfiltrated leaves, 5-10 μg was run on SDS-PAGE, blotted on PVDF membrane, and detected with anti-Myc antibodies (Genscript, #A00704 www.genscript.com) and ECL Plus chemiluminescent substrate (Thermo Fisher Scientific, www.thermofisher.com) as described in (Roberts et al., 2019a). Get A Quote

摘要

Plants mount defense responses by recognizing indications of pathogen invasion, including microbe-associated molecular patterns (MAMPs). Flagellin from the bacterial pathogen Pseudomonas syringae pv. tomato (Pst) contains two MAMPs, flg22 and flgII-28, that are recognized by tomato receptors Flagellin sensing 2 (Fls2) and Flagellin sensing 3 (Fls3), respectively. It is unknown to what degree each receptor contributes to immunity and if they promote immune responses using the same molecular mechanisms. Characterization of CRISPR/Cas9-generated Fls2 and Fls3 tomato mutants revealed that the two receptors contribute equally to disease resistance both on the leaf surface and in the apoplast. However, striking... More

关键词

plant immunity; flagellin; PRR-triggered immunity; bacterial speck disease; tomato