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Matrix metalloproteinase 14 participates in corneal lymphangiogenesis through the VEGF-C/VEGFR-3 signaling pathway

Exp Ther Med. 2017-08-01; 
Hai-Tao Du, Ping Liu
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Monoclonal Antibody Services … UK.). β-actin was used as loading control. The antibody was mouse anti-mouse β-actin monoclonal antibody (1:1,000; A00702-100; GenScript, Nanjing, China). Corneal immunofluorescence microscopy and quantification. The … Get A Quote

摘要

The aim of the present study was to investigate the roles of matrix metalloproteinase 14 (MMP-14) in corneal inflammatory lymphangiogenesis. The expression of MMP-14 was detected by immunohistochemistry, reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and western blot assays, under various corneal conditions. pCMV-MMP-14 or empty pCMV vectors were injected into mouse corneal stroma, 3 days after suture placement in a standard suture-induced inflammatory corneal neovascularization assay. The outgrowth of blood and lymphatic vessels and macrophage recruitment were analyzed using immunofluorescence. The expression levels of vascular endothelial growth factor (VEGF) subtypes were tested by R... More

关键词

cornea inflammation, hemangiogenesis, lymphangiogenesis, matrix metalloproteinase-14, membrane type 1-matrix metalloproteinase