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Human islets contain a subpopulation of glucagon-like peptide-1 secreting α cells that is increased in type 2 diabetes

Mol Metab. 2020; 
Scott A Campbell, Dominic P Golec, Matt Hubert, Janyne Johnson, Nicole Salamon, Amy Barr, Patrick E MacDonald, Koenraad Philippaert, Peter E Light
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Molecular Biology Reagents … Exendin-9 (9-39) salt was purchased from Bachem, reconstituted in PBS, and used at 100 nM concentration for perifusion experiments. IL-1β (Genscript, Z02922-10) was reconstituted in PBS and used at 50 ng/ml for islet culture experiments. Flow cytometry … Get A Quote

摘要

objectives: Our study shows that glucagon-like peptide-1 (GLP-1) is secreted within human islets and may play an unexpectedly important paracrine role in islet physiology and pathophysiology. It is known that α cells within rodent and human pancreatic islets are capable of secreting GLP-1, but little is known about the functional role that islet-derived GLP-1 plays in human islets. methods: We used flow cytometry, immunohistochemistry, perifusions, and calcium imaging techniques to analyse GLP-1 expression and function in islets isolated from cadaveric human donors with or without type 2 diabetes. We also used immunohistochemistry to analyse GLP-1 expression within islets from pancreatic biopsies obtained from... More

关键词

Alpha-cell, Beta-cell, GLP-1, Glucagon, Glucagon-like peptide-1, Human islets