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The Retropepsin-Type Protease APRc as a Novel Ig-Binding Protein and Moonlighting Immune Evasion Factor of

MBio. 2021-12; 
Pedro Curto, Andreia Barro, Carla Almeida, Ricardo S Vieira-Pires, Isaura Simões
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Catalog Antibody … Three microliters of each sample was applied in a 10% polyacrylamide gel and then evaluated by Western blot analysis with the monoclonal mouse anti-human IgG Fc (HRP) (A01854-200; GenScript) (1:20,000 in TBS-T, 2% BSA) … Get A Quote

摘要

Rickettsiae are obligate intracellular Gram-negative bacteria transmitted by arthropod vectors. Despite their reduced genomes, the function(s) of the majority of rickettsial proteins remains to be uncovered. APRc is a highly conserved retropepsin-type protease, suggested to act as a modulator of other rickettsial surface proteins with a role in adhesion/invasion. However, APRc's function(s) in bacterial pathogenesis and virulence remains unknown. This study demonstrates that APRc targets host serum components, combining nonimmune immunoglobulin (Ig)-binding activity with resistance to complement-mediated killing. We confirmed nonimmune human IgG binding in extracts of different rickettsial species and intact ba... More

关键词

APRc, Rickettsia, aspartic protease, evasin, immune evasion, nonimmune immunoglobulin-binding, retropepsin, serum resistance