黄金城集团

至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Quantitative probing of glycosylated queuosine modifications in tRNA

Methods Enzymol. 2021-07; 
Wen Zhang, Tao Pan
Products/Services Used Details Operation
Recombinant Proteins … Elsevier. Methods in Enzymology. Volume 658, 2021, Pages 73-82. Methods in Enzymology … 2.3.10 Incubate the membrane with streptavidin-HRP conjugate (Genscript, M00091) in 30 mL hybridization buffer (1:5000–1:10000 dilution) for 30 min at room temperature. 2.3.11 … Get A Quote

摘要

Queuosine (Q) in humans is a microbiome-dependent modification in the wobble anticodon position of tRNA, tRNA, tRNA, and tRNA. These tRNAs share a 343536 anticodon consensus. In humans, the Q base in tRNA and tRNA is further glycosylated to generate galactosyl-Q (galQ) and mannosyl-Q (manQ) modifications. Q-tRNA modification is known to regulate translation in a codon dependent manner, but the function of Q glycosylation is unknown. A sensitive and quantitative detection method for Q-glycosylation in tRNA is essential to investigate its biological function. Although LC/MS was used in the characterization of glyco-Q tRNA, the requirements of large amount of input material and LC/MS expertise limit its applicatio... More

关键词

Galactosyl-queuosine, Mannosyl-queuosine, Queuosine, tRNA