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Circular RNA circRHOBTB3 represses metastasis by regulating the HuR-mediated mRNA stability of PTBP1 in colorectal cancer

Theranostics. 2021-06; 
Jiaxin Chen, Yizheng Wu, Xin Luo, Dongai Jin, Wei Zhou, Zhenyu Ju, Di Wang, Qing Meng, Huijuan Wang, Xiaotian Fu, Jianbin Xu, Zhangfa Song
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Recombinant Antibody Expression … HCT116 or HEK-293T whole-cell lysates were prepared and incubated with 5 µg of anti-HuR antibody or anti-FLAG/DYKDDDDK (20543-1-AP, Proteintech) antibody controlled by normal rabbit IgG (A01008, GenScript) at 4 C overnight. After treatment with proteinase K buffer, … Get A Quote

摘要

Tumor metastasis of colorectal cancer (CRC) is the main cause of death in most patients and the major difficulty in comprehensive CRC treatment. Circular RNAs (circRNAs) affect many biological functions in solid tumors. However, their mechanisms in CRC metastasis remain unclear. RNA sequencing (RNA-seq) and quantitative real-time PCR were performed to screen differentially expressed circRNAs between CRC tissues and adjacent normal tissues. CCK-8, cell migration and wound healing assays were performed to determine the functions of circRHOBTB3 in cell proliferation and metastasis. RNA pulldown and RNA immunoprecipitation assays were performed to verify the interaction between circRHOBTB3 and the HuR (ELAVL1) pro... More

关键词

ADARB2, FUS, HuR, PTBP1, cancer metastasis, circRHOBTB3, colorectal cancer