| Products/Services Used | Details | Operation |
|---|---|---|
| Polyclonal Antibody Services | … The method of equal quality and equal quantity was adopted when the proteins (10 μl, 30 μg) were separated by electrophoresis on 4–20% or 12% SurePAGE™ Bis–Tris Protein Gels (GenScript, Nangjing, China, M00656/M0066) and transferred onto the polyvinylidene fluoride … | Get A Quote |
Excessive mitophagy plays a role in neuronal death in spinal cord injury (SCI), its molecular regulation remains largely unknown. The present study aims to determine the role of NIX, a member of a unique subfamily of death-inducing mitochondrial proteins, in the regulation of mitophagy in SCI. Here we show that NIX is highly upregulated in SCI and hypoxia, and localized to mitochondria. The mitochondria-bound NIX interacts with autophagosome-localized LC3 (Microtubule-associated protein 1 light chain 3) to form a mitochondria-NIX-LC3-autophagosome complex, resulting in excessive mitophagy in SCI. Downregulation of NIX by RNA interference restores the function of mitochondria in spinal cord neurons under hypoxia... More
