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An insecticide target in mechanoreceptor neurons

Sci Adv. 2022-11; 
Xiaomu Qiao , Xiaoyu Zhang , Zhendong Zhou , Lei Guo , Weiping Wu , Suhan Ma , Xinzhong Zhang , Craig Montell , Jia Huang
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Gene Synthesis A 110-bp ssODN (single-strand oligodeoxynucleotide) was synthesized (GenScript, Nanjing, China) as the donor template to replace the targeted genomic region. After growth for 6 hours at 37°C, cells were collected, and MpNaam was purified using glutathione resin(GenScript, L00206). The purified sample was shipped to GenScript to generate the rabbit polyclonal antibodies. Total protein extracts (10 g) were separated by SDS-PAGE (8 to 16%; GenScript, M00658) and transferred to polyvinylidene difluoride membranes (Millipore, ISEQ00010) using the eBlot L1 wet protein transfer system (GenScript). Get A Quote
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摘要

Hundreds of neurotoxic insecticides are currently in use. However, only a few direct targets have been identified. Here, using Drosophila and the insecticide flonicamid, we identified nicotinamidase (Naam) as a previous unidentified molecular target for an insecticide. Naam is expressed in chordotonal stretch-receptor neurons, and inhibition of Naam by a metabolite of flonicamid, TFNA-AM (4-trifluoromethylnicotinamide), induces accumulation of substrate nicotinamide and greatly inhibits negative geotaxis. Engineered flies harboring a point mutation in the active site show insecticide resistance and defects in gravity sensing. Bees are resistant to flonicamid because of a gene duplication, resulting in the gener... More

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