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Sall4 guides p53-mediated enhancer interference upon DNA damage in mouse embryonic stem cells

Stem Cells. 2022-08; 
Lei Wang, Xiaojun Tan, Lu Chen, Sisi Xu, Weiping Huang, Nan Chen, Yizhou Wu, Chunyan Wang, Daqiang Zhou, Mangmang Li
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Catalog Antibody … A total of 5 μg of antibody and 50 μl Protein G beads (Genscript, L00664-25) were used to immunoprecipitate the DNA-protein complex. After washing 5 times with Wash buffer (50 mM … Get A Quote

摘要

p53 plays pivotal roles in maintaining the genomic stability of mouse embryonic stem cells (mESCs) through transcriptionally activating and repressing target genes. However, how p53 recognizes its repressed targets remains largely unknown. Herein, we demonstrate that Sall4 negatively regulates DNA damage induced apoptosis (DIA) of mESCs through mediating p53 recruitment to enhancers of ESC-associated genes repressed by p53 from promoters of p53-activated genes. Upon DNA damage, Sall4 is transcriptionally repressed by p53, and plays an anti-apoptotic role without altering p53 activation. Moreover, Sall4 is identified as a novel p53-interacting partner. Consistently, Sall4 exerts its anti-apoptotic function in a ... More

关键词

DNA damage, Sall4, enhancer interference, mouse embryonic stem cells, p53