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NBS1 is required for SPO11-linked DNA double-strand break repair in male meiosis

Cell Death Differ. 2020-07; 
Bin Zhang , Zhenghui Tang , Lejun Li , Lin-Yu Lu
Products/Services Used Details Operation
Catalog Antibody The following antibodies were purchased: anti-hNBS1 (Proteintech, 55025-1-AP), anti-mNBS1 (Abcam, ab32074), anti-TDP2 (Proteintech, 12203-1-AP), anti-α-tubulin (Genscript, A01410-100), anti-PLZF (R&D, AF1356), antiγH2AX (Millipore, 05-636), mouse-anti-SYCP3 (Santa cruz, sc-74569), rabbit-anti-SYCP3 (Proteintech, 23024-1- AP), anti-SYCP1 (Novus, NB300-229SS), anti-RPA2 (Abcam, ab2175), anti-RAD51 (Santa Cruz, sc-8349), antiDMC1 (Proteintech, 13714-1-AP). Get A Quote

摘要

DNA double-strand breaks (DSBs) pose a serious threat to genomic stability. Paradoxically, hundreds of programed DSBs are generated by SPO11 in meiotic prophase, which are exclusively repaired by homologous recombination (HR) to promote obligate crossover between homologous chromosomes. In somatic cells, MRE11-RAD50-NBS1 (MRN) complex-dependent DNA end resection is a prerequisite for HR repair, especially for DSBs that are covalently linked with proteins or chemicals. Interestingly, all meiotic DSBs are linked with SPO11 after being generated. Although MRN complex's function in meiotic DSB repair has been established in lower organisms, the role of MRN complex in mammalian meiotic DSB repair is not clear. Here,... More

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