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Enhancing antibody responses by multivalent antigen display on thymus-independent DNA origami scaffolds

Nat Commun. 2024-01; 
Eike-Christian Wamhoff , Larance Ronsard , Jared Feldman , Grant A Knappe , Blake M Hauser , Anna Romanov , James Brett Case , Shilpa Sanapala , Evan C Lam , Kerri J St Denis , Julie Boucau , Amy K Barczak , Alejandro B Balazs , Michael S Diamond , Aaron G Schmidt , Daniel Lingwood , Mark Bathe
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PCR Cloning and Subcloning … L00351) were purchased from GenScript. 96- and 384-well cell culture plates were purchased from Corning and Greiner, respectively Get A Quote

摘要

Protein-based virus-like particles (P-VLPs) are commonly used to spatially organize antigens and enhance humoral immunity through multivalent antigen display. However, P-VLPs are thymus-dependent antigens that are themselves immunogenic and can induce B cell responses that may neutralize the platform. Here, we investigate thymus-independent DNA origami as an alternative material for multivalent antigen display using the receptor binding domain (RBD) of the SARS-CoV-2 spike protein, the primary target of neutralizing antibody responses. Sequential immunization of mice with DNA-based VLPs (DNA-VLPs) elicits protective neutralizing antibodies to SARS-CoV-2 in a manner that depends on the valency of the antigen dis... More

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