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Estrogen receptor β activation inhibits colitis by promoting NLRP6-mediated autophagy

Cell Rep. 2022-10; 
Wentao Fan, Chenchen Ding, Shuhui Liu, Xiaona Gao, Xiaofei Shen, Marthe De Boevre, Zhangshan Gao, Mengcong Li, Shuo Zhang, Yufan Miao, Wenxian Guan, Guangliang Liu, Liping Yan, Sarah De Saeger, Suquan Song
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Proteins, Expression, Isolation and Analysis … ), 3-MA (HY-19312, MedChemExpress), and IL-1b (Z02985-10, Genscript) or TNF-a (Z03333-10, Genscript) were studied on the colitis model mice by intraperitoneal injection for 5 days. … Get A Quote

摘要

Estrogen receptor β (ERβ) and NOD-like receptor family pyrin domain containing 6 (NLRP6) are highly expressed in intestinal tissues. Loss of ERβ and NLRP6 exacerbate colitis in mouse models; however, the underlying mechanisms are incompletely understood. Here, we report that ERβ directly activates the NLRP6 gene expression via binding to estrogen responsive element of Nlrp6 gene promoter. ERβ also physically interacts with the NLRP6 nucleotide-binding domain and promotes NLRP6 inflammasome assembly. The ERβ-NLRP6 axis then interacts with multiple autophagy-related proteins, including ULK1, BECN1, ATG16L1, LC3B, and p62, and affects the autophagosome biogenesis and autophagic flux. Finally, NLRP6-mediated ... More

关键词

CP: Immunology, NLRP6 inflammasome, autophagy, colitis, estrogen receptor β