黄金城集团

至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Heterologous booster vaccination enhances antibody responses to SARS-CoV-2 by improving Tfh function and increasing B-cell clonotype SHM frequency

Frontiers in Immunology. 2024-06; 
Yanli Song, Jiaolei Wang, Zhihui Yang, Changgui Li
Products/Services Used Details Operation
Catalog Peptides Isolated B cells and Tfh cells were cocultured as previously described with minor modifications (23, 24). 3×10^5/well B cells were cultured with or without 1.5×10^5/well Tfh cells in the presence of 5mg/ml spike peptide pools (GenScript, cat RP30020) Get A Quote

摘要

Heterologous prime-boost has broken the protective immune response bottleneck of the COVID-19 vaccines. however, the underlying mechanisms have not been fully elucidated. Here, we investigated antibody responses and explored the response of germinal center (GC) to priming with inactivated vaccines and boosting with heterologous adenoviral-vectored vaccines or homologous inactivated vaccines in mice. Antibody responses were dramatically enhanced by both boosting regimens. Heterologous immunization induced more robust GC activation, characterized by increased Tfh cell populations and enhanced helper function. Additionally, increased B-cell activation and antibody production were observed in a heterologous regimen... More

关键词

homologous, heterologous, SARS-CoV-2, Libra-seq, SHMS, Germinal Center, Tfh cells