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Stiffness-dependent dynamic effect of inflammation on keratocyte phenotype and differentiation

Biomed Mater . 2023-04; 
Jialin Chen 1 2 3 4, Qingyun Mo 1 2, Renwang Sheng 1 2, Qiuzi Long 2 5, Zhixuan Chen 1 2, Chuanquan Liu 1 2, Aini Zhang 1 2, Yifan Luo 1 2, Jia Liu 1 2, Wei Zhang 1 2 3 4
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Recombinant Proteins To evaluate the effect of IL-1β on the phenotype of HTK cells and keratocyte differentiation of hPDLSCs, cells were treated with or without 5 ng ml−1 IL-1β (Genscript Biotech, Z02985). Get A Quote

摘要

Although extensive studies have evaluated the regulation effect of microenvironment on cell phenotype and cell differentiation, further investigations in the field of the cornea are needed to gain sufficient knowledge for possible clinical translation. This study aims to evaluate the regulation effects of substrate stiffness and inflammation on keratocyte phenotype of corneal fibroblasts, as well as the differentiation from stem cells towards keratocytes. Soft and stiff substrates were prepared based on polydimethylsiloxane. HTK and stem cells were cultured on these substrates to evaluate the effects of stiffness. The possible synergistic effects between substrate stiffness and inflammatory factor IL-1βwere ex... More

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