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Caveolin-3 negatively regulates endocytic recycling of cardiac K channels

Am J Physiol Cell Physiol. 2023-09; 
Jian-Yi Huo, Yu-Long Feng, Yue-Tong Chen, Bo Yang, Ya-Ting Zhi, Hao-Jie Wang, Hua-Qian Yang
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Gene Synthesis … 4HA/6myc, Flag-SUR2A-Cav3-FL- 91 6myc/4HA/KEHR-A/3C-S, and Flag-SUR2A-Cav3-MAD1-KEHR-A/3C-S, were generated by GenBuilder 92 (L00701 Genscript) Gibson cloning … Get A Quote

摘要

Sarcolemmal ATP-sensitive potassium (K) channels play a vital role in cardioprotection. Cardiac K channels are enriched in caveolae and physically interact with the caveolae structural protein caveolin-3 (Cav3). Disrupting caveolae impairs the regulation of K channels through several signaling pathways. However, the direct functional effect of Cav3 on K channels is still poorly understood. Here, we used the cardiac K channel subtype, Kir6.2/SUR2A, and showed that Cav3 greatly reduced K channel surface density and current amplitude in a caveolae-independent manner. A screen of Cav3 functional domains revealed that a 25 amino acid region in the membrane attachment domain of Cav3 is the minimal effective segment (... More

关键词

Caveolin-3, KATP channel, Rab11A, cardioprotection, surface density