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Development of a therapeutic monoclonal antibody against circulating adipocyte fatty acid binding protein to treat ischaemic stroke

Br J Pharmacol. 2023-12; 
Boya Liao, Shilun Yang, Leiluo Geng, Jiuyu Zong, Zixuan Zhang, Mengxue Jiang, Xue Jiang, Simeng Li, Aimin Xu, Junlei Chang, Ruby Lai Chong Hoo
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Proteins, Expression, Isolation and Analysis … protein G beads (GenScript, Cat # L00209, China) according to the manufacturer’s manual. … Our protein-protein docking analysis revealed that binding of 6H2 to A-FABP likely did not … Get A Quote

摘要

objective: Adipocyte fatty acid-binding protein (A-FABP) exacerbates cerebral ischaemia injury by disrupting the blood-brain barrier (BBB) through inducing expression of MMP-9. Circulating A-FABP levels positively correlate with infarct size in stroke patients. We hypothesized that targeting circulating A-FABP by a neutralizing antibody would alleviate ischaemic stroke outcome. methods: Monoclonal antibodies (mAbs) against A-FABP were generated using mouse hybridoma techniques. Binding affinities of a generated mAb named 6H2 towards various FABPs were determined using Biacore. Molecular docking studies were performed to characterize the 6H2-A-FABP complex structure and epitope. The therapeutic potential and saf... More

关键词

adipocyte fatty acid binding protein, antibody drug candidate, ischemic stroke, neutralizing monoclonal antibody 6H2