Species |
SARS-CoV-2 |
Protein Construction |
Expressed with the mutations of E484K, K417N, N501Y.
Spike RBD (Arg319-Ser591) Accession # P0DTC2 |
Avi |
Poly-His |
N-term |
|
C-term |
|
Purity |
> 90% as analyzed by SDS-PAGE |
Endotoxin Level |
< 0.2 EU/μg of protein by gel clotting method |
Biological Activity |
SARS-CoV-2 Spike protein (RBD, E484K, K417N, N501Y, His & Avi Tag) can bind with human ACE2 (Cat. No.: Z03484) in functional ELISA assay. |
Expression System |
293 Cells |
Theoretical Molecular Weight |
30 kDa |
Formulation |
Supplied as a solution in PBS, pH 7.4. |
Concentration |
Please refer to the COA for the specific lot. |
Storage & Stability |
Upon receiving, this product remains stable for up to 6 months at -20°C or below. Avoid repeated freeze-thaw cycles. |
![SARS-CoV-2 Spike Protein (RBD, E484K, K417N, N501Y, His & Avi Tag) SARS-CoV-2 Spike Protein (RBD, E484K, K417N, N501Y, His & Avi Tag)](/product/documents/down?doc_name=Z03537_bioactivity Data-ELISA.jpg&file=scm_files/productFile_notes/2021/05/21/2f9a853e-1d73-4775-a82d-b3965037c30e.jpg)
Immobilized ACE-2 Fc Chimera, Human (Cat. No. Z03484) at 2 μg/mL can bind SARS-CoV-2 Spike protein (RBD, E484K, K417N, N501Y, His & Avi Tag) (Cat. No. Z03537) with a serial dilution.
THETM His Tag Antibody [HRP], mAb, Mouse (Cat.No.A00612) is used as a secondary antibody (0.2 μg/mL).
![SARS-CoV-2 Spike Protein (RBD, E484K, K417N, N501Y, His & Avi Tag) SARS-CoV-2 Spike Protein (RBD, E484K, K417N, N501Y, His & Avi Tag)](/product/documents/down?doc_name=Z03537 _SDS-PAGE.jpg&file=scm_files/productFile_notes/2021/05/21/215d281f-a2f9-48c7-9b5a-a13fcd1b0a14.jpg)
Lane 1: 1μg of SARS-CoV-2 Spike protein (RBD, E484K, K417N, N501Y, His & Avi Tag), reducing(R)
> 95% as analyzed by SDS-PAGE
![SARS-CoV-2 Spike Protein (RBD, E484K, K417N, N501Y, His & Avi Tag) SARS-CoV-2 Spike Protein (RBD, E484K, K417N, N501Y, His & Avi Tag)](/product/documents/down?doc_name=Z03537 _Bioactivity-BLI.jpg&file=scm_files/productFile_notes/2022/09/30/a3cd1a46-7edd-47ad-8556-1e263246b21d.jpg)
Loaded ACE-2 Fc Chimera, Human (CHO-expressed) Protein (Cat. No. Z03516) on Protein A Biosensor, can bind SARS-CoV-2 Spike protein (RBD, N501Y, His & Avi Tag) (Cat. No. Z03537) with an affinity constant of 4.44 nM as determined in BLI assay.
Target Background |
SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as 2019-nCoV (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold to severe diseases. SARS-CoV-2 Spike Protein is composed of S1 domain and S2 domain. S1 contains a receptor-binding domain (RBD) that can specifically bind to angiotensin-converting enzyme 2 (ACE2), the receptor on target cells. It is believed that SARS-CoV-2 Spike Protein (RBD) has potential value for the diagnosis of the virus. |
Synonyms |
Lineage B.1.351; Beta variant; 20H/501Y.V2 |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.