Species |
SARS-CoV-2 |
Protein Construction |
Expressed with the mutations of E484K, K417N, N501Y.
Spike RBD (Arg319-Ser591) Accession # P0DTC2 |
Avi |
Poly-His |
N-term |
|
C-term |
|
Purity |
> 90% as analyzed by SDS-PAGE |
Endotoxin Level |
< 0.2 EU/μg of protein by gel clotting method |
Biological Activity |
SARS-CoV-2 Spike protein (RBD, E484K, K417T, N501Y, Avi & His Tag) can bind with human ACE2 in functional ELISA assay. |
Expression System |
CHO |
Theoretical Molecular Weight |
33.7 kDa |
Formulation |
Supplied as a solution in PBS, pH 7.4. |
Concentration |
Please refer to the COA for the specific lot. |
Storage & Stability |
Upon receiving, this product remains stable for up to 6 months at -20°C or below. Please avoid repeated freeze-thaw cycles. |
![SARS-CoV-2 Spike Protein (RBD, E484K, K417T, N501Y, Avi & His Tag) SARS-CoV-2 Spike Protein (RBD, E484K, K417T, N501Y, Avi & His Tag)](/product/documents/down?doc_name=Z03600.jpg&file=scm_files/productFile_notes/2021/05/18/45ec9387-d1c1-46b0-a87b-4151ab2f7dcb.jpg)
Immobilized ACE-2 Fc Chimera, Human at 2 μg/mL can bind SARS-CoV-2 Spike protein (RBD, E484K, K417T, N501Y, Avi & His Tag) (Cat. No. Z03600) with a serial dilution.
THETM His Tag Antibody [HRP], mAb, Mouse(Cat.No.A00612) is used as a secondary antibody.
Target Background |
SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as 2019-nCoV (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold to severe diseases. Lineage P.1, also known as the Brazil(ian) variant is one of the variants of SARS-CoV-2. This variant has 17 amino acid changes, ten of which are in its spike protein, including these three designated to be of particular concern: N501Y, E484K and K417T. SARS-CoV-2 Spike protein (RBD, E484K, K417T, N501Y, Avi & His Tag) carries a Avi Tag followed by a polyhistidine tag at the C-terminus. It has high binding affinity to human chimeric ACE2. |
Synonyms |
Lineage P.1; 20J/501Y.V3; VOC-202101/02; Brazil(ian) variant; Gamma variant |
References |
1. 2. 3. |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.