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Nickel Nanoparticle-Doped Paper as a Bioactive Scaffold for Targeted and Robust Immobilization of Functional Proteins.

ACS Nano.. 2014-06;  8(6):6221-31
BodelÓn G, Mourdikoudis S, Yate L, Pastoriza-Santos I, PÉrez-Juste J, Liz-MarzÁn LM. Bionanoplasmonics Laboratory, CIC biomaGUNE, Paseo de MiramÓn 182, 20009 Donostia-San SebastiÁn, Spain.
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摘要

Cellulose-based materials are widely used in analytical chemistry as platforms for chromatographic and immunodiagnostic techniques. Due to its countless advantages (e.g., mechanical properties, three-dimensional structure, large surface to volume area, biocompatibility and biodegradability, and high industrial availability), paper has been rediscovered as a valuable substrate for sensors. Polymeric materials such as cellulosic paper present high protein capture ability, resulting in a large increase of detection signal and improved assay sensitivity. However, cellulose is a rather nonreactive material for direct chemical coupling. Aiming at developing an efficient method for controlled conjugation of cellulose-... More

关键词

nickel nanoparticles; filter paper; bioconjugation; polyhistidine tag; protein G; immuno-capture; Salmonella; enzyme immobilization