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Puf3 participates in ribosomal biogenesis in malaria parasites.

J. Cell. Sci.. 2018-03; 
LiangXiaoying,HartKevin J,DongGang,SiddiquiFaiza A,SebastianAswathy,LiXiaolian,AlbertIstvan,MiaoJun,LindnerScott E,CuiLi
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… Western blotting was performed using a standard procedure with rabbit anti-Protein C (1:1000, GenScript, Cat# A00637) and rabbit anti-PfAldolase (1:3000, kindly provided by Dr … using the anti-Protein C antibodies (1:1000, GenScript, Cat# A00637) and …

摘要

In this study, we characterized the Puf family gene member Puf3 in the malaria parasites and Secondary structure prediction suggested that the RNA-binding domains of the Puf3 proteins consisted of 11 pumilio repeats that were similar to those in the human Puf-A (also known as PUM3) and Puf6 proteins, which are involved in ribosome biogenesis. Neither (Pf) nor (Py) could be genetically disrupted, suggesting they may be essential for the intraerythrocytic developmental cycle. Cellular fractionation of PfPuf3 in the asexual stages revealed preferential partitioning to the nuclear fraction, consistent with nuclear localization of PfPuf3::GFP and PyPuf3::GFP as detected by immunofluorescence. Furthermor... More

关键词

Plasmodium,Puf protein,RNA-binding protein,Ribosomal biogenesis,Translational regula