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Multiple pathways facilitate the biogenesis of mammalian tail-anchored proteins.

J. Cell. Sci.. 2017; 
Casson Joseph,McKenna Michael,Ha?denteufel Sarah,Aviram Naama,Zimmerman Richard,High Ste
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Proteins, Expression, Isolation and Analysis ). Immunodepletion of TRC40 Protein-A–Sepharose (40 μl bead volume; Genscript, L00210) that had been pre Get A Quote

摘要

Tail-anchored (TA) proteins are transmembrane proteins with a single C-terminal transmembrane domain, which functions as both their subcellular targeting signal and membrane anchor. We show that knockout of TRC40 in cultured human cells has a relatively minor effect on endogenous TA proteins, despite their apparent reliance on this pathway These findings support recent evidence that the canonical TRC40 pathway is not essential for TA protein biogenesis We therefore investigated the possibility that other ER-targeting routes can complement the TRC40 pathway and identified roles for both the SRP pathway and the recently described mammalian SND pathway in TA protein biogenesis. We conclude that, although T... More

关键词

Endoplasmic reticulum,Membrane protein,Protein translocation,SND