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The Auxiliary Calcium Channel Subunit α2δ4 Is Required for Axonal Elaboration, Synaptic Transmission, and Wiring of Rod Photoreceptors.

Neuron. 2017; 
Wang Y, Fehlhaber KE, Sarria I, Cao Y, Ingram NT, Guerrero-Given D, Throesch B, Baldwin K, Kamasawa N, Ohtsuka T, Sampath AP, Martemyanov KA.
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PCR Cloning and Subcloning , 2013 N/A Mouse anti-GAPDH Millipore Cat#AB2302; RRID: AB_11211911 Rabbit anti-c-myc GenScript Cat#A00172; RRID: AB_914457 Mouse anti-CtBP2 Mouse anti-PKCa BD Biosciences Cat#612044; RRID: AB_399431 Abcam Cat#ab11723; RRID: AB_298510 Mouse anti-GPR179 Rabbit anti-Gao Primm Biotech Cat#Ab887; RRID: AB_10792445 Santa Cruz Cat#K-20; RRID: AB_2314438 Goat anti-Arrestin-C Santa Cruz Cat#I-17; RRID: AB_2060084 Rabbit anti-cone arrestin Rabbit anti-Gat1 Millipore Cat#AB15282; RRID: AB_1163387 Santa Cruz Cat#K-20; RRID: AB_2294749 Peanut Agglutinin (PNA) Alexa Fluor 647 conjugate Life Technologies Cat#L32460 Rabbit anti-PSD95 Cell Signaling Technology Cat#3450S; RRID: AB_2292883 Chicken anti-RFP Rockland Cat#RL600-901-379; RRID: AB_10704808 Bacterial and Virus Strains Stellar Competent Cells Clontech Laboratories Cat#636763 Chemicals, Peptides, and Recombinant Proteins Lipofectamine LTX Invitrogen Cat#15338100 Protein G agarose GE Healthcare Biosciences Cat#17-0618-01 Critical Commercial Assays In-Fusion HD Cloning kit Takara Bio USA Cat#638910 pcDNA3.... Commercial antibodies used included: mouse anti-GAPDH (AB2302, Millipore), rabbit anti-c-myc (A00172, Genscript), mouse anti-CtBP2 (612044, BD Biosciences), mouse anti-PKCa (ab11723; Abcam), mouse anti-GPR179 (Primm Biotech; Ab887), rabbit anti-Gao (K-20, Santa Cruz), goat anti-Arrestin-C (I-17, Santa Cruz), rabbit anti-cone arrestin (AB15282, Millipore), rabbit anti-Gat1 (K-20, Santa Cruz), Peanut Agglutinin (PNA) Alexa Fluor 647 conjugate (Life Technologies), rabbit anti-PSD95 (D27E11, Cat# 3450S, Cell Signaling Technology), and chicken anti-RFP (Rockland). Get A Quote

摘要

Neural circuit wiring relies on selective synapse formation whereby a presynaptic release apparatus is matched with its cognate postsynaptic machinery. At metabotropic synapses, the molecular mechanisms underlying this process are poorly understood. In the mammalian retina, rod photoreceptors form selective contacts with rod ON-bipolar cells by aligning the presynaptic voltage-gated Ca2+ channel directing glutamate release (CaV1.4) with postsynaptic mGluR6 receptors. We show this coordination requires an extracellular protein, α2δ4, which complexes with CaV1.4 and the rod synaptogenic mediator, ELFN1, for trans-synaptic alignment with mGluR6. Eliminating α2δ4 in mice abolishes rod synaptogenesis and synapti... More

关键词

calcium channels; cell adhesion; circuit formation; retina; ribbon synapse; synaptic transmission; synaptogenesis; trans-synaptic interactions; vision; wiring