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T-complex protein 1-ring complex enhances retrograde axonal transport by modulating tau phosphorylation.

Traffic. 2018; 
Chen XQ, Fang F, Florio JB, Rockenstein E, Masliah E, Mobley WC, Rissman RA,, Wu C,.
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Gene Synthesis The samples were separated on 4-10% SDS-PAGE gel, transferred, probed with GFP (sc-9996), CCT8 (sc-13891), CDK5 (sc-173), p35 (sc-820), ac-tubulin (6-11B-1) (Santa Cruz Biotechnology), AT8 (MN1020), -tubulin (DM1A) (62204) (Invitrogen), pT231 (55313), pT181 (54960) (ANASPEC), pS199 (A00894), tau (A01387), Avi (A00674), His (A00174) (GenScript), mCherry (GTX59788) (GeneTex), CCT2 (WH0010576M1) (Sigma), actin (60008-1-Ig) (Proteintech), CCT4 (EPR8495), CCT5 (EPR7562) (Epitomics), GSK3 (12456), pGSK3(Ser9) (9323) (Cell Signaling), pGSK3(Tyr216) (13A) (BD), HA. Get A Quote

摘要

The cytosolic chaperonin T-complex protein (TCP) 1-ring complex (TRiC) has been shown to exert neuroprotective effects on axonal transport through clearance of mutant Huntingtin (mHTT) in Huntington's disease. However, it is presently unknown if TRiC also has any effect on axonal transport in wild-type neurons. Here, we examined how TRiC impacted the retrograde axonal transport of brain-derived neurotrophic factor (BDNF). We found that expression of a single TRiC subunit significantly enhanced axonal transport of BDNF, leading to an increase in instantaneous velocity with a concomitant decrease in pauses for retrograde BDNF transport. The transport enhancing effect by TRiC was dependent on endogenous tau expres... More

关键词

TRiC/CCT chaperonin; axonal transport; hyperphosphorylation; tau