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GmYSL7 controls iron uptake, allocation, and cellular response of nodules in soybean

J Integr Plant Biol. 2022-12; 
Xinying Wu, Yongliang Wang, Qiaohan Ni, Haizhen Li, Xuesong Wu, Zhanxin Yuan, Renhao Xiao, Ziyin Ren, Jingjing Lu, Jinxia Yun, Zhijuan Wang, Xia Li
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Proteins, Expression, Isolation and Analysis … For the DNA–protein pulldown assay, the biotinylated fragments were incubated with streptavidin MagBeads (GenScript, L00424) in TES buffer (10 mM Tris-HCl pH 7.5, 1 mM EDTA pH … Get A Quote

摘要

Iron (Fe) is essential for DNA synthesis, photosynthesis and respiration of plants. The demand for Fe substantially increases during legumes-rhizobia symbiotic nitrogen fixation because of the synthesis of leghemoglobin in the host and Fe-containing proteins in bacteroids. However, the mechanism by which plant controls iron transport to nodules remains largely unknown. Here we demonstrate that GmYSL7 serves as a key regulator controlling Fe uptake from root to nodule and distribution in soybean nodules. GmYSL7 is Fe responsive and GmYSL7 transports iron across the membrane and into the infected cells of nodules. Alterations of GmYSL7 substantially affect iron distribution between root and nodule, resulting in d... More

关键词

GmYSL7, GmbHLH300, iron transport, nodule, soybean, symbiosis