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Siglec15 promotes the migration of thyroid carcinoma cells by enhancing the EGFR protein stability

Glycobiology. 2023-07; 
Sijing Huang, Zhi Ji, Jinqiang Xu, Yuerong Yang, BingRui Wu, Qihang Chen, Shuting Geng, Yu Si, Jiayue Chen, Yuanyan Wei, Cong Wang, Zhilong Ai, Jianhai Jiang
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Proteins, Expression, Isolation and Analysis … The supernatant was added with Protein G resin (L00209, Genscript, China) and rotated at … binding proteins. The cleared cell lysis was incubated with mFc or Siglec15-mFc protein … Get A Quote

摘要

purpose: Sialic acid-bound immunoglobulin-like lectin 15 (Siglec15) has emerged as a novel therapeutic target in tumor immunotherapy. This study is designed to investigate the function and mechanism of Siglec15 in thyroid carcinoma (THCA). materials and methods: The information on patients with THCA from TGCA and GEO database were used to analyze the expression of Siglec15 in THCA. THCA cells were treated with Siglec15-mFc, a recombinant fusion protein consisting of the extracellular domain of human Siglec15 and murine IgG Fc. THP-1 cells expressing human Siglec15 and its mutant were co-cultured with THCA cells to mimic the contact between Siglec15-expressing tumor-associated macrophages and THCA cells. Wound-h... More

关键词

EGFR, Siglec15, migration, thyroid carcinoma