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Artificial Extracellular Vesicles Generated from T Cells Using Different Induction Techniques

Biomedicines. 2024-04; 
Ekaterina A Zmievskaya, Sabir A Mukhametshin, Irina A Ganeeva, Elvina M Gilyazova, Elvira T Siraeva, Marianna P Kutyreva, Artur A Khannanov, Youyong Yuan, Emil R Bulatov
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Catalog Antibody … The β-actin antibody (A00730, GenScript, Piscataway, NJ, USA) was used at a dilution of 1:2000 in 5% milk-PBS-Tween for staining for 1.5 h at room temperature. The membranes were … Get A Quote

摘要

Cell therapy is at the forefront of biomedicine in oncology and regenerative medicine. However, there are still significant challenges to their wider clinical application such as limited efficacy, side effects, and logistical difficulties. One of the potential approaches that could overcome these problems is based on extracellular vesicles (EVs) as a cell-free therapy modality. One of the major obstacles in the translation of EVs into practice is their low yield of production, which is insufficient to achieve therapeutic amounts. Here, we evaluated two primary approaches of artificial vesicle induction in primary T cells and the SupT1 cell line-cytochalasin B as a chemical inducer and ultrasonication as a physi... More

关键词

T cells, artificial vesicles, extracellular vesicles, ultrasonication, vesicle induction