| Specificity |
GenScript Streptavidin Antibody is specific to streptavidin. |
| Host Species |
Rabbit |
| Immunogen |
Purified recombinant streptavidin |
| Conjugate |
Unconjugated |
|
Working concentrations for specific applications should be determined by the investigator.The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors.The suitability of this antibody for applications other than those listed below has not been determined.The following concentration ranges are recommended starting points for this product. |
| Application |
Recommended Usage |
| ELISA |
0.5-1 µg/ml |
| Dot blot |
0.5-1 µg/ml |
| Western Blot |
0.5-1 µg/ml |
|
| Form |
Lyophilized |
| Storage Buffer |
Lyophilized in PBS, pH7.4, containing 0.02% sodium azide |
| Reconstitution |
Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 5 mg/ml. |
| Storage Instructions |
The lyophilized product remains stable for up to 1 year at -20 °C from the date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles. |
| Purification |
Protein A/G affinity column |
| Isotype |
Rabbit IgG |
| Clonality |
Polyclonal |
| Clone ID |
Not applicable |
| Note |
Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. |
| Target Background |
Streptavidin is a tetrameric protein from Streptomyces avidinii that binds very tightly to the vitamin biotin with a Kd of ≈10-14 mol/L.The high-affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.GenScript Streptavidin Antibody is developed in rabbit using purified Streptavidin and highly purified from rabbit antiserum by protein G resin. |
| Synonyms |
Rabbit Anti-Streptavidin pAb;Anti Streptavidin |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
