| Specificity |
THE™ Anti-GST Monoclonal Antibody (Mouse) recognizes GST localized at
the C-terminal, N-terminal, and internal region of GST-tagged
fusion proteins.
|
| Host Species |
Mouse |
| Immunogen |
Recombinant GST protein (26 kDa)
|
| Conjugate |
Unconjugated |
Working concentrations for specific applications should be determined by
the investigator. The appropriate concentrations may be affected by secondary
antibody affinity, antigen concentration, the sensitivity of the method of
detection, temperature, the length of the incubations, and other factors. The
suitability of this antibody for applications other than those listed below has
not been determined. The following concentration ranges are recommended
starting points for this product.
|
| Application |
Recommended Usage |
| ELISA |
0.05-0.2 µg/ml |
| Western Blot |
0.1-1.0 µg/ml |
| Immunoprecipitation (IP) |
1 µg/ml |
|
| Form |
Lyophilized |
| Storage Buffer |
1: 40
µg, 100 µg, 200 µg (GenScript, A00865), lyophilized with PBS, pH 7.4,
containing 0.02% sodium azide.
2: 500 µg (GenScript,
A00865), lyophilized with PBS, pH 7.4, without sodium azide or carrier protein. |
| Reconstitution |
Reconstitute the lyophilized powder with deionized water (or equivalent)
to a final concentration of 0.5 mg/mL.
|
| Storage Instructions |
The lyophilized product remains stable for up to 1 year at -20°C from the
date of receipt. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C.
For long term storage, aliquot and store at -20°C or below. Avoid repeated
freezing and thawing cycles.
|
| Purification |
Protein A affinity column |
| Isotype |
Mouse IgG1,κ |
| Clonality |
Monoclonal |
| Clone ID |
2F10B9 |
| Note |
GenScript can offer this product per customer's request including
product size, buffer components, etc.
|
Figure 1. The affinity of THE™ GST Antibody, mAb, Mouse (GenScript, A00865) for C-terminal, N-terminal, and internal GST-tagged fusion proteins was analyzed by Western blotting.
Lane 1: N-terminal GST-tagged fusion protein
Lane 2: Internal GST-tagged fusion protein
Lane 3: C-terminal GST-tagged fusion protein
The primary antibody was THE™ GST Antibody, mAb, Mouse (GenScript, A00865) used at 1 μg/mL concentration. The secondary antibody was Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00160, 1:20,000).
| Target Background |
Schistosoma japonicum glutathione
S-transferase (GST) is a 26 kDa enzyme that conjugates reduced glutathione to
hydrophobic electrophiles. GST is an ideal
fusion partner for production of foreign proteins in commercially available
expression systems because it can be expressed in high levels in E.coli. The fusion proteins can be
purified to homogenity in a single step as the GST portion of the protein binds
tightly to immobilized glutathione. Elution can be accomplished using free
glutathione. The GST portion of the fusion protein can be separated from the
protein of interest by using site-specific proteases. Antibodies directed
against GST can be helpful in detecting the fusion protein during purification
and to detect cleavage of GST from the protein of interest.
|
| Synonyms |
Mouse Anti-GST monoclonal antibody
|
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
