| Specificity |
GenScript THE™ His Tag Antibody [FITC], mAb, Mouserecognizes C-terminal, N-terminal, and internal His tagged fusion proteins. |
| Host Species |
Mouse |
| Immunogen |
A synthetic peptide HHHHHH coupled to KLH |
| Conjugate |
Fluorescein (FITC) |
|
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. |
| Application |
Recommended Usage |
| Immunocytochemistry/Immunofluorescence (ICC/IF) |
0.1-2 µg/ml |
| Flow Cytometry |
0.01-0.1 µg/ml |
|
| Form |
Lyophilized |
| Storage Buffer |
lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide |
| Reconstitution |
Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/mL. |
| Storage Instructions |
The lyophilized product remains stable for up to 1 year at -20 °C from the date of receipt. Upon reconstitution, ti can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles. |
| Purification |
Protein A affinity column |
| Isotype |
Mouse IgG1,κ |
| Clonality |
Monoclonal |
| Clone ID |
6G2A9 |
![THE™ His Tag Antibody [FITC], MAb, Mouse](/product/documents/down?doc_name=12115_20100308203036.JPG&file=scm_files/productFile_notes/2023/11/14/368eb468-88e1-4f11-99ca-497b278d5ce2.JPG "THE™ His Tag Antibody [FITC], MAb, Mouse")
Flow cytometric analysis of transfected HEK293 cell
expressing His-tagged protein using THETM
Anti-His-tag [FITC] Monoclonal Antibody (GenScript, A01620;
shaded histogram) or with a negative control antibody (open
histogram).
| Target Background |
GenScript THE™ His Tag Antibody [FITC], mAb, Mouse is an FITC conjugated THE™ His Tag Antibody, mAb, Mouse (A00186) purified from mice ascites by Protein A affinity column. This product is suitable for detecting the expression level of His fusion proteins by various immunoassays.Absorption Wavelength: 492 nmEmission Wavelength: 518 nmFluorochrome/Protein Ratio: >3 moles FITC per mole antibody |
| Synonyms |
THE™ His Tag Antibody [FITC], mAb, Mouse; |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
