| Specificity |
GenScript THE™ DYKDDDDK Tag Antibody [FITC], mAb, Mouse recognizes C-terminal, N-terminal, and internal DYKDDDDK tagged fusion proteins. |
| Host Species |
Mouse |
| Immunogen |
A synthetic peptide DYKDDDDK coupled to KLH |
| Conjugate |
Fluorescein (FITC) |
|
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. |
| Application |
Recommended Usage |
| Immunocytochemistry/Immunofluorescence (ICC/IF) |
0.5-2 µg/ml |
| Flow Cytometry |
0.5-2 µg for 1 x 106 cells |
|
| Form |
Lyophilized |
| Storage Buffer |
lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide |
| Reconstitution |
Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/mL. |
| Storage Instructions |
The lyophilized product remains stable for up to 1 year at -20 °C from the date of receipt. Upon reconstitution, ti can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles. |
| Purification |
Protein A affinity column |
| Isotype |
Mouse IgG2b,κ |
| Clonality |
Monoclonal |
| Clone ID |
5A8E5 |
| Note |
Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. |
![THE™ DYKDDDDK Tag Antibody [FITC], MAb, Mouse](/product/documents/down?doc_name=12129_20100308210651.JPG&file=scm_files/productFile_notes/2023/11/14/9edc9e63-483a-4877-bac9-8e3e670da199.JPG "THE™ DYKDDDDK Tag Antibody [FITC], MAb, Mouse")
Flow cytometric analysis of transfected HEK-293 cell expressing DYKDDDDK-tagged protein using
THETM Anti-DYKDDDDK [FITC] Monoclonal Antibody (GenScript, A01632; shaded histogram) or with a
negative control antibody (open histogram).
| Target Background |
GenScript THE™ DYKDDDDK Tag Antibody [FITC], mAb, Mouse is FITC conjugated THE™ DYKDDDDK Tag Antibody, mAb, Mouse (A000187) purified from mice ascites by Protein A affinity column. This product is suitable for detecting the expression level of DYKDDDDK fusion proteins by various immunoassays. Absorption Wavelength: 492 nmEmission Wavelength: 518 nmFluorochrome/Protein Ratio: >3 moles FITC per mole antibody |
| Synonyms |
THE™ Anti-DYKDDDDK [FITC] Monoclonal Antibody (Mouse); THE™ Anti-Flag [FITC] Monoclonal Antibody (Mouse); |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
