| Specificity |
The product is specific for Tocilizumab. The antibody is recommended as a detection antibody in a pharmacokinetic (PK) bridging assay with capture antibody GenScript, A01978-40, Anti-Tocilizumab Antibody (14B10), mAb, Mouse. |
| Host Species |
Mouse |
| Immunogen |
Tocilizumab |
| Conjugate |
Unconjugated |
|
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. |
| Application |
Recommended Usage |
| ELISA |
0.01-0.1 µg/ml |
| Competitive ELISA |
1-30 µg/ml |
|
| Form |
Lyophilized |
| Storage Buffer |
Lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. |
| Reconstitution |
Reconstitute the lyophilized powder with deionized water (or equivalent) to a final concentration of 0.5 mg/ml. |
| Storage Instructions |
The lyophilized product remains stable up to 1 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid freeze/thaw cycles. |
| Purification |
Protein A affinity column |
| Isotype |
Mouse IgG2b |
| Clonality |
Monoclonal |
| Clone ID |
6C10 |
| Note |
GenScript can customize this product per customer's request including product size, buffer components, etc. |
ELISA binding of Anti-Tocilizumab Antibody (6C10) (GenScript, A01979-40) with Tocilizumab. While the antibody does not recognize the human IgG (data not shown).Coating antigen: Tocilizumab, 1 µg/ml.Anti-Tocilizumab Antibody (6C10) (GenScript, A01979-40) dilutions start from 1,000 ng/ml.EC₅₀= 1.837 ng/ml.
Standard curve of Tocilizumab Sandwich ELISA. The Tocilizumab Sandwich ELISA assay is developed by using Anti-Tocilizumab Antibody (14B10), mAb, Mouse (GenScript, A01978-40) and Anti-Tocilizumab Antibody (6C10), mAb, Mouse (GenScript, A01979-40) as the capture and detection antibodies, respectivelyIn this ELISA assay, Anti-Tocilizumab Antibody (6C10), mAb, Mouse (GenScript, A01979-40) was labeled with Biotin. GenScript can provide customized conjugation services for this product per customer's request.The sensitivity is less than 100 pg/ml.
Anti-Tocilizumab Antibody (6C10) (GenScript, A01979-40) blocks Tocilizumab binding with Human IL-6R (Z03136, IL-6R, Human).
Coating antigen: IL-6R, 0.5 µg/ml
Tocilizumab-biotin final concentration: 0.5 µg/ml
Anti-Tocilizumab Antibody (6C10) (GenScript, A01979-40) dilutions start from 10 μg/ml
IC₅₀= 0.656 μg/ml
| Target Background |
Tocilizumab also known as atlizumab, is a humanized monoclonal antibody that is approved by the U.S. Food and Drug Administration. It has a high affinity to IL-6R and blocks the IL-6 signaling by preventing the formation of the IL-6/IL-6R complex. Tocilizumab is used for the treatment of rheumatoid arthritis (RA) and systemic juvenile idiopathic arthritis, a severe form of arthritis in children.Anti-Tocilizumab Antibody (6C10), mAb, Mouse is produced from a hybridoma resulting from the fusion of partner and B-lymphocytes obtained from a mouse immunized with Tocilizumab. |
| Synonyms |
Mouse monoclonal to Actemra / RoActemra |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
