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目录产品 » His tag Antibody (C157), mAb, Mouse
His Tag Antibody (C157), MAb, Mouse

ELISA binding of His tag Antibody (C157) (GenScript, A02003) with His-tag fusion protein.
Coating antigen: His-tagged protein, 1 μg/ml.
Antibody dilutions: Start from 1,000 ng/ml.
EC₅₀= 4.694 ng/ml.

His Tag Antibody (C157), MAb, Mouse

Immunoprecipitation (IP) assay of His tag Antibody (C157)(GenScript, A02003) with cell lysate transfected with His-tag fusion protein.
Loading:
Lane 1: Cell lysate
Lane 2: IP with mouse IgG; 1:200
Lane 3: IP with His tag Antibody (C157); 1:200
Lane M: Protein Marker
Primary Antibody:
MonoRab™ Anti-His Tag (C-term) Antibody(25B6E11), mAb,Rabbit (GenScript, A01857-40, 0.5 μg/ml);
Secondary Antibody:
Goat anti-rabbit IgG (H&L) [IRDye800] (Licor,926-32211),0.125 μg/ml

His Tag Antibody (C157), MAb, Mouse

Western blot analysis of His tag Antibody (C157) (GenScript, A02003) with His tag fusion protein Loading:
Lane M: Protein Marker;
Lane 1: 100 ng His tag fusion protein
Lane 2: 50 ng His tag fusion protein
Lane 3: 25 ng His tag fusion protein
Lane 4: 10 ng His tag fusion protein
Primary Antibody:
His tag Antibody (C157) (GenScript, A02003); 1:1000
Secondary Antibody:
Goat anti-Mouse IgG (H&L) [IRDye⁸⁰⁰] (Licor,926-32211) ;0.125 μg/ml

His tag Antibody (C157), mAb, Mouse

A His tag is an amino acid motif in proteins that consists of at least six histidine. It is also known as hexa histidine-tag, 6xHis-tag, His6 tag and polyhistidine-tag. GenScript His tag Antibody (C157), mAb, Mouse is specific to His tag fusion proteins. This antibody can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify and detect His-tagged fusion proteins in bacteria, insect cells, and mammalian cells.
A02003
¥1200

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Specificity The product is specific for His tag fusion proteins.
Host Species Mouse
Immunogen A synthetic peptide HHHHHH coupled to KLH

Application Recommended Usage
ELISA 1: 2,000-1:10,000
Western Blot 1:1,000-1: 5,000
Immunoprecipitation (IP) 1: 200-1:1,000
Other applications User-optimized

Concentration 1 mg/ml, supplied as a suspension in TBS with 50% glycerol and 0.02% sodium azide.
Purification Protein A affinity column
Clone ID C157

  • His Tag Antibody (C157), MAb, Mouse
  • His Tag Antibody (C157), MAb, Mouse

    ELISA binding of His tag Antibody (C157) (GenScript, A02003) with His-tag fusion protein.
    Coating antigen: His-tagged protein, 1 μg/ml.
    Antibody dilutions: Start from 1,000 ng/ml.
    EC₅₀= 4.694 ng/ml.

  • His Tag Antibody (C157), MAb, Mouse
  • His Tag Antibody (C157), MAb, Mouse

    Immunoprecipitation (IP) assay of His tag Antibody (C157)(GenScript, A02003) with cell lysate transfected with His-tag fusion protein.
    Loading:
    Lane 1: Cell lysate
    Lane 2: IP with mouse IgG; 1:200
    Lane 3: IP with His tag Antibody (C157); 1:200
    Lane M: Protein Marker
    Primary Antibody:
    MonoRab™ Anti-His Tag (C-term) Antibody(25B6E11), mAb,Rabbit (GenScript, A01857-40, 0.5 μg/ml);
    Secondary Antibody:
    Goat anti-rabbit IgG (H&L) [IRDye800] (Licor,926-32211),0.125 μg/ml

  • His Tag Antibody (C157), MAb, Mouse
  • His Tag Antibody (C157), MAb, Mouse

    Western blot analysis of His tag Antibody (C157) (GenScript, A02003) with His tag fusion protein Loading:
    Lane M: Protein Marker;
    Lane 1: 100 ng His tag fusion protein
    Lane 2: 50 ng His tag fusion protein
    Lane 3: 25 ng His tag fusion protein
    Lane 4: 10 ng His tag fusion protein
    Primary Antibody:
    His tag Antibody (C157) (GenScript, A02003); 1:1000
    Secondary Antibody:
    Goat anti-Mouse IgG (H&L) [IRDye⁸⁰⁰] (Licor,926-32211) ;0.125 μg/ml


Target Background A His tag is an amino acid motif in proteins that consists of at least six histidine. It is also known as hexa histidine-tag, 6xHis-tag, His6 tag and polyhistidine-tag. GenScript His tag Antibody (C157), mAb, Mouse is specific to His tag fusion proteins. This antibody can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify and detect His-tagged fusion proteins in bacteria, insect cells, and mammalian cells.
Synonyms Mouse monoclonal to His tag

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