Figure 1. The potency of Anti-Pertuzumab Antibody (1A3) (GenScript, A02150-40) at blocking Pertuzumab binding with Human HER2 recombinant protein was tested using ELISA. The coating antigen was Pertuzumab at 1 µg/mL concentration. The final concentration of HER2 recombinant protein was 200 ng/mL. The anti-Pertuzumab antibody dilutions started from 30 μg/mL. IC50= 0.387 µg/mL was observed.
Figure 2. The affinity of binding of Anti-Pertuzumab Antibody (1A3), mAb, Mouse (GenScript, A02150-40) with Pertuzumab was analyzed using ELISA. The coating antigen was Pertuzumab at 1 µg/mL concentration. The Anti-Pertuzumab Antibody (1A3), mAb, Mouse (GenScript, A02150-40) was used at dilutions starting from 1,000 ng/mL.
EC50= 13.1 ng/mL was observed. The Anti-Pertuzumab Antibody (1A3), mAb, Mouse (GenScript, A02150-40) did not recognize human IgG (data not shown).
Figure 4. A standard curve of Pertuzumab Sandwich ELISA was generated. The Pertuzumab Sandwich ELISA assay was developed by using Anti-Pertuzumab Antibody (1A3), mAb, Mouse (GenScript, A02150-40; 1 µg/mL) and Anti-Pertuzumab Antibody (11H4), mAb, Mouse (GenScript, A02151-40; 1 µg/mL) as the capture and detection antibodies, respectively. In this ELISA assay, Anti-Pertuzumab Antibody (11H4), mAb, Mouse (GenScript, A02151-40) was labeled with Biotin. The sensitivity of detecting Pertuzumab is up to 0.499 ng/mL.
GenScript can provide customized conjugation services for this product per the customer's request.
Figure 3. The binding of Anti-Pertuzumab Antibody (1A3), mAb, Mouse (GenScript, A02150-40) with Trastuzumab and Pertuzumab was tested using ELISA. The coating antigen was Pertuzumab at 1 µg/mL concentration, or Trastuzumab at 1 µg/mL concentration. The Anti-Pertuzumab Antibody (1A3), mAb, Mouse (GenScript, A02150-40) was used at dilutions starting from 1,000 ng/mL. The results show that the antibody does not recognize Trastuzumab.
Anti-Pertuzumab Antibody (1A3), mAb, Mouse
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A02150 |
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¥990 |
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联系黄金城集团 |