ELISA binding of MMAE Antibody (69F7), mAb, Mouse (GenScript, A02224) with MMAE, DXd, DM1 and MMAF conjugated antibody respectively.
The coating antibody was MMAE Antibody (69F7), mAb, Mouse (GenScript, A02224) used at 2 μg/mL concentration. The primary antibody were different payload conjugated antibodies, including MMAE, DXd, DM1 and MMAF conjugated antibodies used at 1 μg/mL concentration. The secondary antibody was Mouse Anti-Human IgG Fc Antibody (50B4A9)[HRP], mAb (GenScript, A01854) used at 0.25 μg/ml concentration.
MMAE Antibody (69F7), mAb, Mouse (GenScript, A02224) is specific to MMAE and has no cross-reactivity with MMAF, DXd, and DM1.
![MMAE Antibody (69F7), MAb, Mouse MMAE Antibody (69F7), MAb, Mouse](/product/documents/down?doc_name=A02224_MMAE MRD.jpg&file=scm_files/productFile_notes/2023/08/29/efd5e0ca-7fff-4388-8d15-57c76a4f585b.jpg)
MRD analysis of the detection of MMAE conjugated antibody in biological matrix.
The MRD is the minimum dilution necessary for the detection of MMAE conjugated antibody in biological matrix with least interference. Serum samples from cynomolgus monkey were serially diluted to determine the MRD of this assay, and the test result suggestedthat MRD was as 1:5.
In this ELISA assay, MMAE Antibody (69F7), mAb, Mouse (GenScript, A02224) is used as the capture antibody and Mouse Anti-Human IgG Fc Antibody (50B4A9)[HRP], mAb (GenScript, A01854) is the detection antibody.
![MMAE Antibody (69F7), MAb, Mouse MMAE Antibody (69F7), MAb, Mouse](/product/documents/down?doc_name=A02224_MMAE Sandwich ELISA.jpg&file=scm_files/productFile_notes/2023/08/29/8f7cd2e6-e16a-4fd2-bab2-254722f3929a.jpg)
Standard curve of MMAE conjugated Trastuzumab Sandwich ELISA by anti-idiotype antibody and A02224 (69F7).
In this ELISA assay, MMAE Antibody (69F7), mAb, Mouse (GenScript, A02224) was coated at a concentration of 2 μg/ml, and Anti-Trastuzumab Antibody (15H2), mAb, Mouse (GenScript, A02033) conjugated with HRP was used as a detection antibody at a concentration of 0.2 μg/ml.
In this ELISA assay, a four-parameter logistic curve fitting program was used to create a standard curve with the R-Square equal to 0.99974. The typicaldynamic range of the assay is 1.56-100 ng/mL and its sensitivity of detecting MMAE conjugated Trastuzumab is up to 1.56 ng/ml.
![MMAE Antibody (69F7), MAb, Mouse MMAE Antibody (69F7), MAb, Mouse](/product/documents/down?doc_name=A02224_MMAE Competitive ELISA.jpg&file=scm_files/productFile_notes/2024/06/14/0199d271-9291-47a1-a801-f11da285a89a.jpg)
Standard curve of MMAE Competitive ELISA.
The competitive ELISA assay is developed by using free MMAE block the binding of MMAE Antibody (69F7), mAb, Mouse (GenScript, A02224) to HRP conjugated MMAE.
Capture antibody: MMAE Antibody (69F7), mAb, Mous, 2 μg/ml
Detection reagent: HRP conjugated MMAE, 2 μg/ml
Free MMAE dilution start from 1 μg/ml.
MMAE Antibody (69F7), mAb, Mouse
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A02224 |
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¥1600 |
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