![MMAF Antibody (11B8), MAb, Mouse MMAF Antibody (11B8), MAb, Mouse](/product/documents/down?doc_name=A02225_MMAF Cross-reacitvity Test.jpg&file=scm_files/productFile_notes/2023/06/06/050cba54-3b0a-4e23-aaba-f0fdb9d81000.jpg)
ELISA binding of MMAF Antibody (11B8), mAb, Mouse (GenScript, A02225) with MMAF, MMAE, DXd and DM1 conjugated antibody respectively.
The coating antibody was MMAF Antibody (11B8), mAb, Mouse (GenScript, A02225) used at 2 μg/mL concentration. The primary antibody were different payload conjugated antibodies, including MMAF, MMAE, DXd and DM1 conjugated antibodies used at 1 μg/mL concentration. The secondary antibody was Mouse Anti-Human IgG Fc Antibody (50B4A9)[HRP], mAb (GenScript, A01854) used at 4 μg/ml concentration.
MMAF Antibody (11B8), mAb, Mouse (GenScript, A02225) is specific to MMAF and has no cross-reactivity with MMAE, DXd, and DM1.
![MMAF Antibody (11B8), MAb, Mouse MMAF Antibody (11B8), MAb, Mouse](/product/documents/down?doc_name=A02225_MMAF Competitive ELISA.jpg&file=scm_files/productFile_notes/2023/06/06/451eb76f-348d-4d55-ac2c-f95a0df3f81c.jpg)
Standard curve of MMAF Competitive ELISA.
The competitive ELISA assay is developed by using free MMAF block the binding of MMAF Antibody (11B8), mAb, Mouse (GenScript, A02225) to HRP conjugated MMAF.
Capture antibody: MMAF Antibody (11B8), mAb, Mouse, 2 μg/ml
Detection reagent: HRP conjugated MMAF, 10 μg/ml
Free MMAF dilution start from 2 μg/ml.
![MMAF Antibody (11B8), MAb, Mouse MMAF Antibody (11B8), MAb, Mouse](/product/documents/down?doc_name=A02225_MMAF Sandwich ELISA_Human IgG.jpg&file=scm_files/productFile_notes/2023/06/06/a96b41dc-3c60-4216-b9a7-b591c0d203dd.jpg)
Standard curve of MMAF conjugated Pembrolizumab Sandwich ELISA by anti-Human IgG Fc antibody and A02225.
The Sandwich ELISA assay is developed by using MMAF Antibody (11B8), mAb, Mouse (GenScript, A02225) and Mouse Anti-Human IgG Fc Antibody (50B4A9)[HRP], mAb (GenScript, A01854) as the capture and detection antibodies, respectively.
In this ELISA assay, a four-parameter logistic curve fitting program was used to create a standard curve with the R-Square equal to 0.997.
Capture antibody: MMAF Antibody (11B8), mAb, Mouse, 2 μg/ml
Detection antibody: Mouse Anti-Human IgG Fc Antibody (50B4A9)[HRP], mAb, 4 μg/ml
![MMAF Antibody (11B8), MAb, Mouse MMAF Antibody (11B8), MAb, Mouse](/product/documents/down?doc_name=A02225_MMAF Sandwich ELISA.jpg&file=scm_files/productFile_notes/2023/06/06/acb29c64-e4cd-4082-b367-8cbdc9bcc50a.jpg)
Standard curve of MMAF conjugated Pembrolizumab Sandwich ELISA by anti-idiotype antibody and A02225.
The Sandwich ELISA assay is developed by using MMAF Antibody (11B8), mAb, Mouse (GenScript, A02225) and MonoRab™ Anti-Pembrolizumab Antibody(90G12)[Biotin], mAb, Rabbit (GenScript, A01853) as the capture and detection antibodies, respectively.
In this ELISA assay, a four-parameter logistic curve fitting program was used to create a standard curve with the R-Square equal to 0.99974.
Capture antibody: MMAF Antibody (11B8), mAb, Mouse, 2 μg/ml
Detection antibody: MonoRab™ Anti-Pembrolizumab Antibody(90G12)[Biotin], mAb, Rabbit, 0.05 μg/ml
MMAF Antibody (11B8), mAb, Mouse
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A02225 |
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¥1600 |
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