| Specificity |
This product is specific for scFvs in various species (humanized, mouse), various orders (VH-linker-VL, VL-linker-VH) and various forms of scFv-based bispecific antibodies(DART, BiTE, TandAbs, IgG(H)-scFv2, scFv-Fab-Fc, etc). This product exhibits minimal cross-reactivity with human, mouse, goat, and rabbit immunoglobulins. |
| Host Species |
Rabbit |
| Immunogen |
This information is confidential and belongs to GenScript. |
| Conjugate |
iFluor 647 |
|
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. |
| Application |
Recommended Usage |
| Flow Cytometry |
1 µg/ml-10 µg/ml |
|
| Form |
Liquid |
| Storage Buffer |
Supplied in PBS (pH 7.2), containing 10 mg/ml BSA, 0.02% sodium azide and 50% glycerol. |
| Concentration |
0.5 mg/ml |
| Storage Instructions |
Store at -20°C and it should be protected from prolonged exposure to light. This product is stable for 1 year upon receipt, when handled and stored as instructed. |
| Purification |
Protein A affinity column |
| Isotype |
Rabbit IgG, κ |
| Clonality |
Monoclonal antibody cocktail |
| Note |
GenScript can customize this product per customer's request including product size, buffer components, etc. |
![MonoRab™ Rabbit Anti-scFv Cocktail (Min X) [iFluor 647]](/product/documents/down?doc_name=A02305_Detecting CAR-T Cells.png&file=scm_files/productFile_notes/2024/05/28/72ed8fcf-53ae-452e-8cb0-19f40fa6dc56.png "MonoRab™ Rabbit Anti-scFv Cocktail (Min X) [iFluor 647]")
Flow cytometric analysis of untransduced T cells or CAR-transduced T cells using MonoRab™ Rabbit Anti-scFv Cocktail (Min X) [iFluor 647] (GenScript, A02305).
The results show that MonoRab™ Rabbit Anti-scFv Cocktail (Min X) [iFluor 647] can achieve good cell clustering for CAR-transduced T cells and show minimal non-specificity for untransduced T cells.
![MonoRab™ Rabbit Anti-scFv Cocktail (Min X) [iFluor 647]](/product/documents/down?doc_name=A02305_Detecting ScFv By Flow Cytometric.png&file=scm_files/productFile_notes/2024/05/28/e1f3c60f-3f07-4f25-9e0b-0d4968a1cc11.png "MonoRab™ Rabbit Anti-scFv Cocktail (Min X) [iFluor 647]")
Flow cytometric analysis of Raji cells using anti-CD19 scFv and MonoRab™ Rabbit Anti-scFv Cocktail (Min X) [iFluor 647] (GenScript, A02305) (Red peak) compared to A02305 alone (green).
| Target Background |
A single chain fragment variable (scFv) is a type of recombinant antibody. It is approximately 25 kDa and consists of the variable regions of the heavy (VH) and light (VL) chains of an antibody, which are connected by a flexible peptide linker. scFvs have several advantages, including their small molecular weight, strong penetration, and high specificity. They play crucial roles in targeted therapy, imaging diagnosis, and biological detection. Importantly, in the field of cell therapy, scFvs can act as the antigen recognition domain of CAR-T cells and determine the targeting ability of these cells. |
| Synonyms |
scFv; Single-chain fragment variable; Single chain antibody; Single-chain variable region fragment; Single-chain fv |
For research use only. Not intended for human and animal therapeutic or diagnostic use.
