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Taq DNA Polymerase

Taq DNA Polymerase is a thermostable DNA Polymerase isolated from an E. coli strain that carries the Taq DNA polymerase gene. Taq DNA Polymerase is the most common polymerase used for PCR.Note: This product is supplied with 10X reaction buffer containing 15 mM magnesium chloride. dNTP (10 mM) mixture must be ordered separately (see
E00007
￥160

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Description

Taq DNA Polymerase is a thermostable DNA Polymerase isolated from an E. coli strain that carries the Taq DNA polymerase gene. Taq DNA Polymerase is the most common polymerase used for PCR.

  • Note: This product is supplied with 10X reaction buffer containing 15 mM magnesium chloride. dNTP (10 mM) mixture must be ordered separately (see related products).

    		•
    Note

    Formulation
    GenScript Taq DNA Polymerase has been formulated using GenScript's proprietary technology. The enzyme can be shipped at room temperature or even 37°C for seven days without any loss of activity.
    Unit Definition
    One unit is defined as the amount of enzyme that can incorporates 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C.
    10 X Reaction Buffer (with Mg2+)
    500 mM KCl, 100 mM Tris HCl (pH 9.0 at 25°C), 15 mM MgCl2, 1% Triton X-100
    Buffer. This buffer is optimized for use with 200 µM dNTPs.
    Note: If the reaction is performed without this buffer, then add 0.1% Triton X-100 (final concentration) to ensure high activity.
    Concentration:
    Taq is delivered in 5 units/µl in 20 mM Tris HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 0.1% Triton X-100 and 50% glycerol.

    产品概述
    Key Features
    Key Feature
    • Terminal Transferase Activity: Taq DNA Polymerase has terminal transferase activity which results in the addition of a single nucleotide (adenosine) at the 3' end of the extension product.
    • High-purity: No contamination activity has been detected in standard test reactions.

    产品属性
    QC
    QC Tests
    PCR* performance, activity, nuclease
    Lane Taq Unit
    1Leading
    Brand A    		0.1
    2 0.25
    3 0.5
    4Leading
    Brand B    		0.1
    5 0.25
    6 0.5
    7GenScript0.1
    8 0.25
    9 0.5

    Storage & Stability
    Storage
    Store the product at -20°C.

    * The PCR process is covered by US. Patents Nos. 4683195 and 4683202, issued to Cetus and owned by Hoffman-La Roche Inc. GenScript does not encourage or support the unauthorized use of the PCR process. Use of this product is recommended for persons who either have a license to perform PCR or are not required to obtain a license. Sale of this product is restricted to regions or countries where native Taq DNA polymerase patents have been invalidated.

    应用指导
    Applications
    The applications of Taq DNA Polymerase include the following:
    • PCR*
    • 3'A-tailing of blunt ends
    • Primer extension
    • DNA sequencing

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